瘤体内注射32磷一玻璃微球对裸鼠人肝癌移植瘤的抗癌效应研究

目的研究瘤体内注射32磷-玻璃微球(32P-GMS)对裸鼠人肝癌移植瘤的抗癌作用。方法动态观察治疗组和对照组裸鼠的肿瘤生长状况，分批处死，取瘤体及其邻近组织进行光镜和电镜观察。结果与对照组相比，14d治疗组抑瘤率为59．7％～93．6％(P<0．01)。瘤体吸收剂量为7320Gy，肿瘤细胞可被一次性杀死；吸收剂量为1830～3660Gy时，瘤细胞大多损伤坏死，并出现分化较好的瘤细胞；吸收剂量为366Gy或更低时，仍残存有增殖活跃的瘤细胞。结论瘤体内注射1830～3660Gy的32P-GMS，可对人肝癌移植瘤产生最佳抗癌效应。

Study on the antitumor effect of phosphorus - 32 glass microspheres intrattmoral injected to the implanted human hepatocellular carcinoma mass of nude mice

Objective To study the antitumor effect of phosphorus- 32 glass microspheres (~P-GMS) intratumoral injected to the implanted human hepatocellular carcinoma cell mass of nude mice. Method After dynamically observing the growth of tumor for 3 d～28 d, the experimental fifty- two Balb/c tumor loaded nude female mice in both control and treated group were killed in batches, andthen the tumors and their nearby tissues were examined by light and electron microscopy. Results In comparing with the control group, the treatment group attained the tumor inhibiting rates of 59.7 % ～ 93.6% ( P < 0.01 ) at 14 d. As the mass having the absorbed dose of 7 320 Gy, the tumor cells were completely destroyed. When the absorbed doses ranged from 1 830 Gy to 3 660 Gy, most of the tumor cells showed the evidences of injury or necrosis, but some well- differentiated tumor cell and enhanced effect of the auto - immunocytes ap- peared. As the absorbed dose being 366 Gy or less, some tumor cells still remained their active proliferative ability. Conclusion When32p- GMS of 1 830Gy～ 3 660 Gy were intratumoral injected to the experimental model of implanted hepatocellular carcinoma cells, it pro- duced excellent anticancer effect