商陆正丁醇部位醋制前后肠道毒性变化研究

为探索商陆毒性部位炮制前后对肠道毒性变化的影响,提取分离获得了商陆正丁醇毒性部位,在整体动物模型水平,灌胃给药后观察小鼠肠道肿胀程度,测定小鼠肠道不同部位的含水量及粪便的含水量,考察商陆正丁醇部位炮制前后对腹泻及肠道肿胀的影响;在细胞水平,采用MTT法,测定肠细胞HT-29,IEC-6的IC50,比较醋制前后商陆正丁醇部位的毒性变化。结果显示商陆正丁醇部位能够导致肠道肿胀,表现为引起十二指肠、空肠的水肿,且可导致粪便含水量增加引起腹泻,可抑制肠细胞HT-29,IEC-6增殖,表明其具有肠细胞毒性,对HT-29细胞的IC5014.59 mg·L-1,IEC-6 IC5043.77 mg·L-1;经醋制,小鼠肠道肿胀程度显著减弱,肠道和粪便的含水量明显降低,对HT-29和IEC-6的抑制作用减轻,对HT-29细胞的IC5058.51 mg·L-1,IEC-6 IC5084.37 mg·L-1。由此可知,醋制法具有降低商陆正丁醇部位的毒性作用。

Intestinal toxicity of n-BuOH fraction from Phytolacca Radix before and after being processed with vinegar

To research the intestinal toxicity of n-BuOH fraction in Phytolacca Radix before and after being processed with vinegar. Toxic n-BuOH fractions were separated from Phytolacca Radix. In the animal model, the level of intestinal edema, water content of intestine and stool, IC₅₀ values of HT-29 and IEC-6 were detected with MTT method to compare the changes in toxicity of n-BuOH fractions from Phytolacca Radix before and after being processed with vinegar. n-BuOH fractions of Phytolacca Radix could cause intestinal edema in mice, increase the edema of duodenum, jejunum and the water content in stool, inhibit the proliferation of HT-29 cells and IEC-6 cells, indicating its intestinal toxicity, with HT-29 IC₅₀ at 14.59 mg·L^-1 and IEC-6 IC₅₀ at 43.77 mg·L^-1. After being processed with vinegar, the level of intestinal edema, edema of duodenum and jejunum and the water content in stool and inhibition ratio of cells line were reduced, with HT-29 IC₅₀ at 58.51 mg·L^-1 and IEC-6 IC₅₀ at 84.37 mg·L^-1. After being processed with vinegar, the toxicity of n-BuOH fractions from Phytolacca Radix decreased obviously.