癌症患者外周血核异常淋巴细胞分析

目的观察分析肺癌、肝癌和胃癌患者外周血核异常淋巴细胞,探讨染色体畸变诱发癌症的机制。方法选肺癌、肝癌和胃癌患者为观察对象,以正常人群为对照,通过微核试验观察分析外周血微核细胞和其他核异常淋巴细胞的发生情况,计算核异常淋巴细胞千分率(‰)。结果肺癌(6.61‰,χ~2=52.99,P=0.000 0)、(4.47‰,χ~2=42.96,P=0.000 0)、(6.16‰,χ~2=69.23,P=0.000 0)、(7.55‰,χ~2=62.94,P=0.000 0)、(4.61‰,χ~2=56.53,P=0.000 0)、(5.84‰,χ~2=65.51,P=0.000 0)]、肝癌(6.08‰,χ~2=39.13,P=0.000 0)、(3.75‰,χ~2=23.62,P=0.000 0)、(5.47‰,χ~2=48.34,P=0.000 0)、(7.06‰,χ~2=49.15,P=0.000 0)、(4.31‰,χ~2=46.39,P=0.000 0)、(5.44‰,χ~2=52.71,P=0.000 0)]和胃癌(5.67‰,χ~2=27.52,P=0.000 0)、(3.53‰,χ~2=17.35,P=0.000 0)、(4.73‰,χ~2=27.51,P=0.000 0)、(6.73‰,χ~2=38.39,P=0.000 0)、(3.77‰,χ~2=29.04,P=0.000 0)、(5.23‰,χ~2=43.56,P=0.000 0)]患者外周血淋巴细胞细胞核形态明显异常,微核、核连接、核畸形、核固缩、核崩解、核残迹等核异常淋巴细胞率显著增高,与对照组比较差异具有统计学意义(P<0.01)。结论肺癌、肝癌和胃癌等疾病与染色体畸变或核异常细胞增加有关,核异常细胞可以作为染色体损伤和癌症风险评估的生物标志物。

The analysis of lymphocytes with nuclei abnormalities in peripheral blood of patients with cancers

LI Maojin;WANG Rugang;HU Anyi(Beijing Centers for Disease Control and Prevention / Beijing Centers for Preventive Medicine Research,Beijing 100020,China;Fuwai Hospital Chinese Academy of Medical Sciences / National Center for Cardiovascular Diseases,Beijing 100037,China)