支气管哮喘患儿血清IL-17和IL-36水平及临床意义

目的 探讨支气管哮喘患儿血清白细胞介素-17(IL-17)、白细胞介素-36(IL-36)水平和嗜酸性粒细胞(EOS)计数及临床意义。方法 选取2017年7月-2018年7月在潍坊市人民医院小儿内科门诊就诊及病房住院哮喘儿童65名,其中哮喘急性发作期组儿童30名,缓解期组儿童35名,健康儿童25名作为对照组,采用酶联免疫吸附试验(ELISA)检测各组血清IL-17、IL-36水平,采用血细胞分析仪常规方法检测外周血EOS计数,并进行统计学分析。结果 1)急性发作期组和缓解期组IL-17水平分别为(45.73±8.86)pg/ml和(21.06±5.51)pg/ml,均显著高于对照组(7.29±4.29)pg/ml(P<0.01);且急性发作期组IL-17水平明显高于缓解期组(P<0.01)。2)急性发作期组和缓解期组IL-36水平分别为(53.76±10.60) pg/ml和(24.45±6.59) pg/ml,均显著高于对照组(8.46±4.86) pg/ml(P<0.01);且急性发作期组IL-36水平高于缓解期组,差异有统计学意义(P<0.01)。3)在哮喘患儿中,IL-17和IL-36水平呈正相关(P<0.01);且IL-17和IL-36水平分别与EOS计数呈正相关(P<0.01)。结论 IL-17和IL-36参与儿童哮喘发病过程,其血清水平可作为儿童哮喘诊断和病情评估较好的生物学指标。

Clinical significance and the levels of serum IL-17 and IL-36 in children with bronchial asthma

Objective To analyze serum levels of interleukin-17(IL-17), interleukin-36 (IL-36) and eosinophil (EOS) count in children with bronchial asthma, and to discuss the clinical significance. Methods Totally 65 children with asthma were enrolled from July 2017 to July 2018 in Weifang People′s Hospital, of whom 30 cases were in acute exacerbation, 35 cases were in remission, and 25 health children were as control group. The serum levels of IL-17 and IL-36 were detected by enzyme-linked immunosorbent assay (ELISA), the eosinophil count in peripheral blood was measured by hematology analyzer, and the statistical analysis was performed. Results 1) The serum levels of IL-17 in acute exacerbation group[(45.73±8.86)pg/ml] and remission group[(21.06±5.51)pg/ml] were significantly higher than that in control group[(7.29±4.29)pg/ml] (P<0.01), and serum levels of IL-17 in acute exacerbation group was significantly higher than that in remission group(P<0.01). 2) The serum levels of IL-36 in acute exacerbation group[(53.76±10.60)pg/ml ] and remission group [(24.45±6.59) pg/ml] were significantly higher than that in control group[(8.46±4.86) pg/ml] (P<0.01), and serum level of IL-36 in acute exacerbation group was significantly higher than those in remission group(P<0.01). 3) In asthma children, serum level of IL-17 were positively correlated with the level of IL-36(P<0.01), and significant correlation were noted between the serum levels of IL-17, IL-36 and EOS count (P<0.01). Conclusion IL-17 and IL-36 are all involved in the pathogenesis of serum asthma in children, and serum levels of IL-17 and IL-36 may become a good biomarker for the diagnosis and evaluation of severity of asthma children.