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| 尖锐湿疣、鲍温样丘疹病及鲍温病皮损中人乳头瘤病毒感染与P16蛋白表达的关系 | |
| 引用本文： | 郭书萍,陈丽瑛,白莉,冯艳. 尖锐湿疣、鲍温样丘疹病及鲍温病皮损中人乳头瘤病毒感染与P16蛋白表达的关系[J]. 中华皮肤科杂志, 2002, 35(3): 191-194 |
| 作者姓名： | 郭书萍 陈丽瑛 白莉 冯艳 |
| 作者单位： | 030001,太原,山西医科大学附属第一医院皮肤性病科 |
| 摘 要： | 目的：探讨鲍温样丘疹病（BP）、鲍温病（BD）及尖锐湿疣（CA）皮损中高、低危人乳头瘤病毒（HPV）感染对P16蛋白表达的影响。方法：用荧光定量PCR（FQ－PCR）方法检测30例CA病损及12例正常人皮肤粘膜中HPV6／11、HPV16／18DNA；用原位杂交方法检测30例BP、15例BD中HPV16DNA；用免疫组化方法检测上述组织中P16蛋白、Ki-67蛋白的表达。结果：在HPB感染皮损中，P16蛋白、Ki-67蛋白的表达均增高（P＜0．05），且在HPV16阳性的BP、BD中的表达高于HPV6／11感染的CA（P＜0．05）；P16蛋白表达与Ki-67蛋白表达呈正相关（P＜0．05）。结论：HPV感染皮损中，P16蛋白的过度表达可能是机体对HPV感染引起细胞异常增殖的一种反馈调节；P16蛋白表达的不同可能是由于高、低危HPV引起的Rb蛋白功能失活不同。 |
| 关 键 词： | 尖锐湿疣 鲍温样丘疹病 鲍温病 人乳头状瘤病毒 蛋白质P16 |
| 修稿时间： | 2001-07-09 |
Relationship between Human Papillomavirus Infection and Expression of p16 in the Lesions of Condyloma Acuminata,Bowenoid Papulosis and Bowen′ s Disease |
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| GUOShuping,CHENLiying,BAILi,FENGYan. Relationship between Human Papillomavirus Infection and Expression of p16 in the Lesions of Condyloma Acuminata,Bowenoid Papulosis and Bowen′ s Disease[J]. Chinese Journal of Dermatology, 2002, 35(3): 191-194 | |
| Authors: | GUOShuping CHENLiying BAILi FENGYan |
| Affiliation: | GUOShuping,CHENLiying,BAILi,FENGYan.DepartmentofDematology,TheFirstHospital,ShanxiMedicalUniversity,Taiyuan,030001,China |
| Abstract: | ObjectiveToinvestigatetheeffectofhigh-andlow-riskhumanpapillomavirus(HPVs)ontheexpressionofp16proteininthelesionsofcondylomaacuminata(CA),Bowenoidpapulosis(BP)andBowen'sdisease(BD).MethodsHPV6/11,HPV16/18DNAinthelesionswereexaminedwithFQ-PCRmethodin30casesofCAandinnormalskinormucosaof12cases.HPV16DNAweredetectedwithinsituhybridizationmethodin30casesofBPand15casesofBD.P16,Ki-67proteinswereexaminedwithimmunohistochemicalSPmethodinthesamespecimens.ResultsBothp16proteinandKi-67expressionwereincreasedinHPV-infectedlesions(P<0.05),andtheywerehigherinthehigh-riskHPVinfectedlesionsthanthatinthelow-risk(P<0.05).Theex-pressionofp16proteincorrelatedwiththatofKi-67proteininHPV-positivelesions(P<0.05).ConclusionsTheover-expressionofp16proteininHPV-infectedlesionsmightbeakindoffeed-backmechanismwhichiscausedbyHPV.Thattheexpressionofp16proteininlesionswithhigh-riskHPVsdiffersfromthatoflow-riskHPVsmightbeattributabletothedifferencesinfunctionalinactivationofRbproteinbydifferentHPVs. |
| Keywords: | Condylomataacuminata Bowenoidpapulosis Bowen'sdisease Papillomavirus human Proteinp16 |
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