CD40基因-1C/T多态性与急性冠脉综合症遗传易感性的研究

目的探讨CD40基因-1C/T多态性与急性冠脉综合症(ACS)的关系。方法采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)技术检测127例急性心肌梗死(AMI)患者(AMI组)、162例不稳定心绞痛(UA)患者(UA组)和159名健康体检者(对照组)CD40基因-1C/T的基因型;酶联免疫吸附试验(ELISA)检测可溶性CD40(sCD40)水平。结果 CD40基因-1位点三种基因型及等位基因频率在AMI组、UA组和健康对照组中分布差异均有统计学意义(P=0.001),相对风险分析显示CC基因型患者AMI和UA的风险分别是TT基因型的6.919和3.073倍;携带C等位基因患者AMI和UA的风险分别是T等位基因型的2.421和1.692倍;sCD40水平在ACS组与对照组间、ACS组内不同基因型者间的分布差异均有统计学意义(P<0.01)。结论 CD40基因-1C/T多态性与ACS的发病具有相关性,C等位基因可能是ACS的遗传易感基因。

Study on the gene polymorphisms of CD40-1C/T and acute coronary syndrome

Objective To investigate the association of CD40-1C/T gene polymorphisms with acute coronary syndrome(ACS).Methods Total of 289 patients with ACS were chosen,which including 127 patients with acute myocardial infarction(AMI,AMI group) and 162 patients with unstable angina(UA,UA group),and 159 healthy controls were taken as the control group.The CD40-1C/T gene polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).The serum level of soluble CD40(sCD40) was determined by enzyme-linked immunosorbent assay(ELISA).Automated biochemical analyzer was used to measure the serum lipids level.Results There was a significant difference of genotype distribution among AMI group,UA group and control group(P=0.001),the relative risk suffered from AMI and UA of the people with CC genotype was 6.919 and 3.073 times of the people with TT genotype;the relative risk suffered from AMI and UA of the people with C allele was 2.421 and 1.692 times of the one with T allele;the level of sCD40 was significantly different between ACS group and control group,and also among the three genotypes in ACS groups.Conclusions CD40-1C/T polymorphism is associated with ACS,and C allele may be a genetic risk fators of ACS.