阿托伐他汀对心肌梗死后大鼠主动脉平滑肌NADPH氧化酶的影响

目的探讨心肌梗死后大鼠主动脉平滑肌NADPH氧化酶的变化及阿托伐他汀的干预作用。方法取雄性SD大鼠,通过是否结扎左前降支分为手术组和假手术组,手术组大鼠于术后第二天随机给予阿托伐他汀[10mg/(kg.d)](心梗干预组)或等体积生理盐水(心梗组)灌胃共5周,第6周处死大鼠取胸主动脉平滑肌,采用WST-1法及RT-PCR法分别测定平滑肌超氧阴离子(O2-)浓度及Nox1 mRNA和Nox4 mRNA表达,并探讨其相关性。结果心梗组O2-浓度、Nox1 mRNA及Nox4 mRNA表达高于假手术组(P<0.01)和心梗干预组(P<0.05)。O2-浓度分别与Nox1 mRNA及Nox4 mRNA表达呈正相关(r=0.53,P<0.05;r=0.56,P<0.05)。结论心梗后大鼠平滑肌NADPH氧化酶系统被激活,阿托伐他汀通过降低Nox1 mRNA及Nox4 mRNA表达而减少O2-产生。

Influence of atorvsatin on NADPH oxidase of aortic smooth muscle in rat after myocardial infarction

Objective To explore the change of NADPH oxidese and the intervention effect of atorvsatin on NADPH oxidase of atorvsatin aortic smooth muscle in rats after myocardial infarction(MI).Methods Male Sprague-Dawley rats were divided into operated group and sham group according to ligation or not of anterior descending coronary artery.The operated rats were assigned randomly to intragastrically receive atorvastatin[10 mg/(kg·day)](MI intervention group)or saline(MI group) respectively on the second day after operation for 5 weeks.On the sixth week,the rats were killed and aortic smooth muscle was taken.The concentration of superoxide(O-2) was measured with WST-1 method and the expressions of Nox1 mRNA and Nox4 mRNA were detected with RT-PCR,and the correlations between the concentration of O-2 and the expression of Nox1 mRNA and Nox4 mRNA were respectively calculated.Results The concentration of O-2 and the expression of Nox1 mRNA and Nox4 mRNA in MI group increased significantly compared to sham group(P<0.01) and MI intervention group(P<0.05).The concentration of O-2 positively correlated to the expression of Nox1 mRNA and Nox4 mRNA(r=0.53,P<0.05;r=0.56,P<0.05 respectively).Conclusions The NADPH oxidase system is activated in aortic smooth muscle of rats after MI.Atorvsatin decreases the production of O-2 by inhibiting the expression of Nox1 mRNA and Nox4 mRNA.