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| 内皮祖细胞对狭长窄蒂皮瓣成活面积的影响 | |
| 引用本文： | 余道江,赵天兰,孟庆友,谢晓明,陈琦,韩文雅,伍丽君,徐又佳. 内皮祖细胞对狭长窄蒂皮瓣成活面积的影响[J]. 实用全科医学, 2011, 0(12): 1838-1839,1847,F0003 |
| 作者姓名： | 余道江 赵天兰 孟庆友 谢晓明 陈琦 韩文雅 伍丽君 徐又佳 |
| 作者单位： | 苏州大学附属第二医院整形外科;苏州大学附属第二医院骨科; |
| 基金项目： | 江苏省苏州市青年科教兴卫项目(SWKQ0915) |
| 摘 要： | 目的研究大鼠骨髓来源的内皮祖细胞（EPC）对狭长窄蒂皮瓣成活面积的影响。方法 SD大鼠15只,12只大鼠背部两侧各设计制作1块皮瓣,皮瓣设计为：蒂宽=蒂长=0.8 cm,蒂部所携带直径为3.5 cm的皮瓣,形状类似＂乒乓球拍＂,建立皮瓣的动物模型,随机分为两组：对照组及实验组;另3只大鼠,冲洗骨髓腔,密度离心法获得单个核细胞;在适宜的培养环境中（EGM-2MV培养液）培养出EPC;鉴定细胞CD34、CD133、vWF及VEGFR-2表型,并将目的细注射移植于皮瓣,对实验组及对照组皮瓣进行大体观察,测量皮瓣成活面积,计算局部毛细血管密度。结果注射EPC的皮瓣的存活面积以及毛细血管密度显著高于对照部位（P〈0.05）。结论 EPC移植于狭长窄蒂皮瓣后,加速皮瓣缺血缺氧组织再血管化,改善皮瓣缺血缺氧状态,扩大皮瓣成活面积。 |
| 关 键 词： | 狭长窄蒂皮瓣 内皮祖细胞 成活面积 |
Study on the Survival Area of Narrow Pedicle Flap after Transplanting Endothelial Progenitor Cells |
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| YU Dao-jiang,ZHAO Tian-lan,XU You-jia,et al.. Study on the Survival Area of Narrow Pedicle Flap after Transplanting Endothelial Progenitor Cells[J]. Applied Journal Of General Practice, 2011, 0(12): 1838-1839,1847,F0003 | |
| Authors: | YU Dao-jiang ZHAO Tian-lan XU You-jia et al. |
| Affiliation: | YU Dao-jiang,ZHAO Tian-lan,XU You-jia,et al.Department of Plastic Surgery,the Second Affiliated Hospital of Suzhou University,Suzhou 215004,Jiangsu,China |
| Abstract: | Objective To study the effect of transplanting rat bone marrow derived endothelial progenitor cells to the survival area of narrow pedicle flap.Methods 12 SD rats were divided into 2 groups in random.A flap was designed and put on either side of a rat's back,and the flap was designed in the shape of a table tennis.The width and the length of the flap were both 0.8 cm and the pedicle was with the diameter of 3.5 cm.Bone marrow mononuclear cells were harvested by flushing bone marrow cavity from the other 3 S... |
| Keywords: | Narrow pedicle flap Endothelial progenitor cell Survival area |
| 本文献已被 CNKI 维普 等数据库收录！ | |