鞘内注射昂丹司琼对慢性脊神经炎性痛大鼠的影响

目的探讨鞘内给予5-HT3受体阻滞剂昂丹司琼对慢性脊神经炎性痛大鼠行为学及脊髓背角c-Fos蛋白表达的影响。方法成年雄性SD大鼠48只随机均分为6组（n=8）：空白组（A组）、模型组（B组）、生理盐水组（C组）和昂丹司琼10μg/kg组（D1组）、25μg/kg组（D2组）、50μg/kg组（D3组）。制作大鼠脊神经炎性痛模型后14 d行鞘内注射,并测定大鼠机械缩足反射阈值和热缩足反射潜伏期的变化情况;应用免疫组化技术观察大鼠脊髓c-Fos表达变化情况。结果与B组相比,D2组机械缩足反射阈值、热缩足反射潜伏期明显增高、脊髓背角中c-Fos蛋白表达显著减少（P〈0.01）,D1组与B组比较,无明显变化（P〉0.05）;D2与D3组间差异无统计学意义（P〉0.05）。结论鞘内给予昂丹司琼25μg/kg可以减轻大鼠慢性脊神经炎性痛导致的痛觉过敏。

Effect of Intrathecal Administration of Ondansetron on Chronic Spinal Nerve Inflammatory Pain in Rats

Objective To investigate the effects of intrathecal administration of 5-HT3 receptor antagonist Ondansetron on the ethology of rats with spinal nerve chronic inflammatory pain and the expression of c-Fos in the spinal cord.Methods Forty-eight male Sprague-Dawley（SD） rats were randomly allocated into control group（A）,model group（B）,NS group（C）,Ondansetron 10 μg/kg group（D1）,Ondansetron 25 μg/kg group（D2） and Ondansetron 50 μg/kg group（D3）.On the 14th day after model replication,Von Frey test,Hargreaves test and Immunohistochemistry staining were used to investigate the effects of the ehtology of rats and the expression of c-Fos in the spinal cord after intrathecal administration of Ondansetron.Results Compared with the NS group,mechanical withdrawal threshold and withdrawal thermal latenc were increased significantly and the expression of c-Fos in the spinal cord was reduced significantly in the Ondansetron 25 μg/kg group（P0.01）.There were no significant difference between D2 and D3 group（P0.05）.Conclusion Hyperalgesia was reduced significantly by intrathecal administration of 5-HT3 receptor antagonist in rats with spinal nerve chronic inflammatory pain.