蒙医温针对大鼠疲劳模型肝脏MDA、GSH含量及GSH-P_X、SOD活性的影响

目的:通过对自由基代谢的影响,探讨蒙医温针疗法对疲劳大鼠的作用机理。方法:选用SD雄性大鼠,分为正常对照组、模型组和温针组,每组11只。采用21 d力竭游泳方式制作运动性疲劳大鼠模型。温针组取蒙医“顶会”和“心穴”,使用MLY-Ⅰ型蒙医疗术温针仪进行温针,每隔2 d治疗1次,每次15 min,共7次。采用硫代巴比妥酸法测定丙二醛(MDA)含量;采用比色法测定谷胱甘肽(GSH)和谷胱甘肽过氧化物酶(GSH-PX)含量和活性;采用黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)活性。观察末次运动后24 h大鼠肝组织中MDA、GSH、GSH-PX和SOD的变化。结果:①模型组MDA含量与正常对照组相比无显著性差异(P>0.05);温针组MDA含量与模型组相比无显著性差异(P>0.05)。②模型组GSH和GSH-PX含量及活性与正常对照组相比均显著性降低(P<0.01);温针组GSH和GSH-PX含量及活性与模型组相比均明显增高(P<0.05)。③模型组SOD活性与正常对照组相比显著降低(P<0.05);温针组SOD活性与模型组相比显著增高(P<0.01)。结论:蒙医温针疗法可以通过提高肝组织内的抗自由基氧化酶活性,发挥抗疲劳作用。

Effects of Electric Mildly-warmed Needle of Inner Mongolian Medicine on Liver MDA and GSH Content, GSH-Px and SOD Activity in Fatigue Rats

Objective: To investigate the effect of electric mildly-warmed needle of inner Mongolian medicine on changes of free radical metabolism in the liver tissue of rats with fatigue.Methods: A total of 33 male SD rats were evenly randomized into control,model and electro-mildly-warmed needle(EMWN) groups.Fatigue model was established by forcing the rat to swim in a water pool till exhaustion,once daily,continuously for 21 days."Dinghui"(central spot of the bregmatic bone) and "Xinxue"(the center of the depression beneath the 7th thoracic vertebra) were punctured with silver needle which was warmed electrically by using a MLY-I Electrical Needle-warming Apparatus,once every 3 days,7 sessions altogether.After decapitation,the rat's liver was taken,homogenated and centrifuged for detecting malondialdehyde(MDA) content with thio-barbituric acid,reduced glutathione(GSH) contents,glutathione peroxidase(GSH-Px) activity with chromatometry and superoxide dismutase(SOD) activity with xanthine oxidase method respectively.Results: Compared with control group,liver GSH content,GSH-PX and SOD activity in model group lowered significantly(P<0.05,0.01),while in comparison with model group,these 3 indexes of EMWN group increased considerably(P<0.05,0.01).No significant differences were found among 3 groups in MDA levels(P>0.05).The results showed that the effect of mildly-warmed needle treatment could resist fatigue induced decline of activity of antioxidant enzymes.Conclusion: Electric mildly-warmed needle therapy of inner Mongolian medicine can raise the activity of antioxidant enzymes in liver tissue of fatigue rats,which may underlie the action of mildly-warmed needle in improving fatigue.