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| 54例无精子症、少精子症患者Y染色体AZF微缺失的检测 | |
| 引用本文： | 刘晓翌,肖晓素,王勇强,蔡志明. 54例无精子症、少精子症患者Y染色体AZF微缺失的检测[J]. 中国男科学杂志, 2004, 18(4): 36-39 |
| 作者姓名： | 刘晓翌 肖晓素 王勇强 蔡志明 |
| 作者单位： | 北京大学深圳医院生殖中心遗传室,深圳,518036 |
| 摘 要： | 目的 探讨Y染色体上AZF微缺失与男性无精子症及少精子症之间的关系。方法 采用多重PCR技术，对54例无精子症及少精子症患者AZF4个区的15个序列标签位点(STS)进行了微缺失检测，并同时做了细胞遗传学检查。结果 54例患者中共有4例发现微缺失(7．4％)，其中有2例在17例无精子症患者中发现(11．8％)，另2例在37例少精子症患者中发现(5．4％)。结论 AZF微缺失是导致男性无精子及少精子的重要原因之一，细胞遗传学检查与AZF微缺失无相关性。 |
| 关 键 词： | 无精子因子 微缺失 无精子症 少精子症 |
| 修稿时间： | 2004-05-10 |
Detection of AZF microdeletions on Y chromosome in 54 cases of male with azoospermia and oligozoospermia |
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| Liu Xiaoyi,Xiao Xiaosu,Wang Yongqiang,Cai ZhimingGenetic Laboratory,Reproductive Center,Bejing University Shenzhen Hospital,Shenzhen ,China. Detection of AZF microdeletions on Y chromosome in 54 cases of male with azoospermia and oligozoospermia[J]. Chinese Journal of Andrology, 2004, 18(4): 36-39 | |
| Authors: | Liu Xiaoyi Xiao Xiaosu Wang Yongqiang Cai ZhimingGenetic Laboratory Reproductive Center Bejing University Shenzhen Hospital Shenzhen China |
| Affiliation: | Liu Xiaoyi,Xiao Xiaosu,Wang Yongqiang,Cai ZhimingGenetic Laboratory,Reproductive Center,Bejing University Shenzhen Hospital,Shenzhen 518036,China |
| Abstract: | Objective To investigate microdeletions of AZF on Y chromosome in male with azoospermia andoligozoospermia. Methods Multiplex polymerase chain reaction (PCR) analysis and cytogenetic analysis were donein the 54 cases with azoospermia and oligozoospermia. 15 STS primers were used in the four regions of AZF. ResultsFour of 54 cases (7.4%) showed microdeletion of at least one STS. Of these, 2 cases were found in 17 azoospermia and2 cases were found in 37 oligozoospermia. Conclusion AZF microdeletion is one of the most important causes ofazoospermia and oligozoospermia. There is no relativity between cytogenetic analysis and AZF microdeletions. |
| Keywords: | Azoospermia factor (AZF) microdeletion azoospermia oligozoospermia multiplex polymerasechain reaction |
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