| 甘草多糖提取工艺条件的研究 |
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| 引用本文： | 韩荣生,李鹏.甘草多糖提取工艺条件的研究[J].世界中西医结合杂志,2011,6(3):234-237. |
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| 作者姓名： | 韩荣生 李鹏 |
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| 作者单位： | 1. 河南南阳医专第一附属医院,河南,南阳,473068
2. 浙江中医药大学,浙江,杭州,310053 |
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| 摘 要： | 目的在超声条件下提取甘草中有效成分甘草多糖（GPS）并测定其含量,以筛选获得适宜的甘草多糖提取工艺条件。方法采用正交试验设计,选取提取时间、提取温度及料液比作为工艺因素,每个因素采用3个水平,选择L9（34）正交表进行实验,同时用硫酸-苯酚法测定甘草多糖的含量来作为综合评价指标。结果在超声条件下提取甘草多糖的适宜条件为：提取次数为3次,提取时间为20 min,提取温度为45℃,料液比为1∶20（g.mL-1）,该提取条件下甘草多糖的含量为2.39%。结论利用超声提取多糖相对其他提取方法有较大优势,可缩短提取时间、提高提取效率,且操作方便、自动化程度高,便于大规模工业生产,为从药材或制剂中提取甘草多糖提供了依据及参考。
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| 关 键 词： | 甘草多糖 超声提取 正交实验 含量测定 |
Study of the Extraction Technical Conditions of Angelica Polysaccharide |
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| HAN Rong-sheng,Li Peng.Study of the Extraction Technical Conditions of Angelica Polysaccharide[J].World Journal Of Integrated Traditional and Wesrern Medicine,2011,6(3):234-237. |
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| Authors: | HAN Rong-sheng Li Peng |
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| Institution: | HAN Rong-sheng1,Li Peng2(1.No.1 Affiliated Hospital of Henan Nanyang Medical School,Nanyang Henan 473068,2.Zhejiang University of Traditional Chinese Medicine,Hangzhou Zhejiang 310053) |
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| Abstract: | Objective To extract the effective component,glycyrrhiza polysaccharide(GPS)of glycyrrhiza under ultrasonic condition and determine its content so as to acquire the proper extraction technical conditions of GPS.Methods Orthogonal experimental design was adopted.Extraction time,extraction temperature and solid-liquid ratio were taken as technical factors.3 levels were adopted in each factor.L9(34)orthogonal table was selected in the experiment.Sulfuric acid-phenol method was used to measure the content of GP... |
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| Keywords: | Glycyrrhiza Polysaccharide Ultrasonic Extraction Orthogonal Experiment Content Measurement |
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