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| 鸢尾苷元对氧化损伤血管内皮细胞MCP-1和ICAM-1表达的影响 | |
| 引用本文： | 王金凤,张艳萍,杨翠燕,侯明晓,王芳,王国玉,王淑红. 鸢尾苷元对氧化损伤血管内皮细胞MCP-1和ICAM-1表达的影响[J]. 中国中药杂志, 2010, 35(15): 2001-2003 |
| 作者姓名： | 王金凤 张艳萍 杨翠燕 侯明晓 王芳 王国玉 王淑红 |
| 作者单位： | 中国人民解放军第二○八医院心脏介入科,吉林,长春,130062 |
| 基金项目： | 吉林省科技发展计划项目(20050914,20090919) |
| 摘 要： | 目的:观察鸢尾苷元对氧化低密度脂蛋白(ox-LDL)致氧化损伤血管内皮细胞(VEC)的保护作用及对MCP-1和ICAM-1mRNA表达的影响,探讨其抗动脉粥样硬化的作用机制。方法:采用体外培养大鼠VEC,加入ox-LDL建立VEC氧化损伤模型,加入不同剂量的鸢尾苷元,通过MTT比色法观察细胞活力,并采用RT-PCR方法检测VECMCP-1和ICAM-1mRNA的表达情况。结果:鸢尾苷元对ox-LDL所致的VEC损伤具有明显的保护作用,并显著抑制ox-LDL诱导VECMCP-1和ICAM-1mRNA的过度表达。结论:鸢尾苷元抗VEC氧化损伤和抑制MCP-1,ICAM-1的过度表达是其抗动脉粥样硬化的重要作用机制。 |
| 关 键 词： | 鸢尾苷元 血管内皮细胞 氧化低密度脂蛋白 细胞间黏附分子-1 单核细胞趋化蛋白-1 |
| 收稿时间： | 2009-10-09 |
Effect of tectorigenin on MCP-1 and ICAM-1 mRNA expression in injured vascular endothelial cells |
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| WANG Jinfeng,ZHANG Yanping,YANG Cuiyan,HOU Mingxiao,WANG Fang,WANG Guoyu and WANG Shuhong. Effect of tectorigenin on MCP-1 and ICAM-1 mRNA expression in injured vascular endothelial cells[J]. China Journal of Chinese Materia Medica, 2010, 35(15): 2001-2003 | |
| Authors: | WANG Jinfeng ZHANG Yanping YANG Cuiyan HOU Mingxiao WANG Fang WANG Guoyu WANG Shuhong |
| Affiliation: | Cardiac Intervention Department of 208th Hospital of PLA, Changchun 130062, China;Cardiac Intervention Department of 208th Hospital of PLA, Changchun 130062, China;Cardiac Intervention Department of 208th Hospital of PLA, Changchun 130062, China;Cardiac Intervention Department of 208th Hospital of PLA, Changchun 130062, China;Cardiac Intervention Department of 208th Hospital of PLA, Changchun 130062, China;Cardiac Intervention Department of 208th Hospital of PLA, Changchun 130062, China;Cardiac Intervention Department of 208th Hospital of PLA, Changchun 130062, China |
| Abstract: | Objective : To observe the protective effect of tectorigenin on the vascular endothelial cells(VEC) injured by oxidant low density lipoprotein-cholesterol and the expression of MCP-1 and ICAM-1 mRNA, and explore the mechanism of anti-atherosclerosis. Method : The VEC of rat was cultured in vitro and the 100 mg·L-1 ox-LDL inducing oxidant injured model was used in this study. Different dosage tectorigenin was added into VEC and the activity of VEC was observed by MTT colorimetry. The expression of MCP-1 and ICAM-1 mRNA in VEC was detected by RT-PCR. Result : Tectorigenin had significantly protective effect on the VEC injured by ox-LDL and obviously inhibited the excessive expression of MCP-1 and ICAM-1 mRNA in VEC. Conclusion : It was the critical mechanism of anti-atherosclerosis that tectorigenin prevented the VEC oxidant injured and inhibited the excessive expression of MCP-1 and ICAM-1. |
| Keywords: | tectorigenin vascular endothelial cells oxidant low density lipoprotein-cholesterol intercellular adhesion molecule-1 monocyte chemotatic protein-1 |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |