不同培养条件对皮肤角质形成细胞和成纤维细胞增殖的影响

目的观察含不同血清浓度培养基对皮肤角质形成细胞和成纤维细胞体外活性的影响。方法体外培养、扩增皮肤角质形成细胞和成纤维细胞，按3×10^5／ml的密度接种于96孔板，加入含不同血清浓度的K-SFM培养基培养。并用MTY比色法观察细胞活性。结果应用K—SFM＋10％FBS组培养基培养成纤维细胞与DMEM＋10％FBS为对照组培养细胞的活性影响相同（P〉0．05）；应用K-SFM＋1％FBS培养基培养角质形成细胞与K—SFM为对照组培养细胞的活性影响相同（P〉0．05）；结论在构建人工皮肤体外模型时，角质形成细胞和成纤维细胞可分别加用含1％FBS、10％FBS的K—SFM培养基进行培养。

Investigate the activity of skin Keratinocytes and Fibroblasts influenced by Different culture conditions in vitro

Objective To investigate the activity of skin Keratinocytes and Fibroblasts influenced by medium with different concentrations of serum in vitro. Methods Keratinocytes and Fibroblasts were cultured in vitro, inoculating into 96 Orifice in accordance with 3 - 10 ^5 / ml density, applied with K - SFM Media with different serum to culture, and activity of cells observed by the method of MTT colorimetric. Results Application of K - SFM ＋ 10% FBS medium and DMEM ＋ 10 % FBS for the control group to culture. Fibroblasts , the activity of the cells are the same （ P 〉 0.05） ; Application of K - SFM ＋ 1% FBS medium and K - SFM for the control group to culture keratinocytes, the activity of the ceils are the same （ P 〉 0. 05）. Conclusions Construction of artificial skin model in vitro, Keratinocytes and Fibroblasts can be plus1% FBS, 10% FBS to the K- SFM respectively at culture medium .