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| 扶正解毒含药血清对染镍细胞中信号转导通路的影响 | |
| 引用本文： | 王婷,刘志东,陈佑碧. 扶正解毒含药血清对染镍细胞中信号转导通路的影响[J]. 职业与健康, 2011, 27(3): 262-264 |
| 作者姓名： | 王婷 刘志东 陈佑碧 |
| 作者单位： | 广东省惠州市职业病防治院住院部,516008 |
| 摘 要： | 目的通过结晶硫化镍在支气管上皮细胞系（16 HBE）中磷酸化氨基末端蛋白激酶（P-JNK）和P38的表达,探讨镍致癌的分子机制和中草药对职业性肿瘤的防治作用。方法用SDS聚丙烯酰胺凝胶电泳和蛋白免疫印迹杂交（Western blot）法检测16 HBE细胞中P-JNK和P38的表达。结果硫化镍能明显激活P-JNK和P38的表达,硫化镍浓度越高,磷酸化P-JNK和P38的表达值越高,而且存在剂量-效应关系,染镍组与对照组比较,差异有统计学意义（P〈0.05、P〈0.01）。结论结晶型硫化镍激活16 HBE细胞中P-JNK和P38的表达,可能是镍致癌的分子机制之一,扶正解毒含药血清对职业性肿瘤有一定的防治作用。 |
| 关 键 词： | 硫化镍 支气管上皮细胞 P-JNK P38 |
Effect of Fuzheng Jiedu Decoction on Signaling Pathway Induced by Nickel in Human Bronchus Epithelial cells |
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| WANG Ting,LIU Zhi-dong,CHEN You-bi. Effect of Fuzheng Jiedu Decoction on Signaling Pathway Induced by Nickel in Human Bronchus Epithelial cells[J]. Occupation and Health, 2011, 27(3): 262-264 | |
| Authors: | WANG Ting LIU Zhi-dong CHEN You-bi |
| Affiliation: | WANG Ting,LIU Zhi-dong,CHEN You-bi(Huizhou Hospital for Occupational Disease Prevention and Treatment,Guangdong,516008,China) |
| Abstract: | [Objective]to explore the molecular mechanism of nickel carcinogenesis and preventive and therapeutic effect of Chinese herbal medicine on occupational cancer,through the study of nickel purite on expression of P-JNK and P38 in human bronchus epithelial(16 HBE) cells.[Methods]SDS polyacrylamide gel electrophoresis and Western blot hybridization method were adopted to detect the expression of P-JNK and P38.[Results]Nickel sulfide could significantly activate P-JNK and P38expression.The expression value incre... |
| Keywords: | P-JNK P38 |
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