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| 四嗪二甲酰胺对慢性B淋巴细胞白血病CD4+CD25+调节性T细胞体外作用的研究 | |
| 引用本文： | 邱莲女,周永列,黄强,胡惟孝. 四嗪二甲酰胺对慢性B淋巴细胞白血病CD4+CD25+调节性T细胞体外作用的研究[J]. 医学研究杂志, 2011, 40(3): 34-39 |
| 作者姓名： | 邱莲女 周永列 黄强 胡惟孝 |
| 作者单位： | 1. 浙江省人民医院检验中心,杭州,310014 2. 浙江省人民医院血液科,杭州,310014 3. 杭州,浙江工业大学药学院 |
| 基金项目： | 国家自然科学基金资助项目，浙江省医药卫生科学研究基金项目计划，浙江省医学重点学科建设基金 |
| 摘 要： | 目的研究四嗪二甲酰胺(ZGDHu-1)对慢性B淋巴细胞白血病(chronic B cell lymphocytic leukemia,B-CLL)CD4+CD25+调节性T细胞(regulatory T cells,Tregs)的影响,初步探讨其对CD4+CD25+Tregs的作用机制。方法 B-CLL外周血淋巴细胞分离培养,流式细胞术检测ZGDHu-1作用前后CD4+CD25+/highCD127low、CD19+CD5+以及CD4+CD25+Tregs细胞活力、细胞凋亡、Fas、FasL、APO2.7及Bcl-2、Bax。结果 B-CLL的CD4+CD25+/highCD127low、CD19+CD5+明显高于正常对照组,且CD4+CD25+/highCD127low与CD19+CD5+明显正相关。ZGDHu-1作用后,B-CLL的CD4+CD25+/highCD127low、CD19+CD5+明显降低,CD4+CD25+Tregs活力明显下降和凋亡明显升高;CD4+CD25-T细胞活力也有一定程度的下降,凋亡升高,但其凋亡明显低于CD4+CD25+Tregs。ZGDHu-1作用后,CD4+CD25+Tregs的APO2.7明显增加,Bcl-2明显降低,Bax没有变化,而CD4+CD25-T细胞FasL明显增加。结论 ZGDHu-1作用后B-CLL的CD4+CD25+Tregs明显下降,可能与Bcl-2/Bax比值降低,APO2.7表达增高,而促进B-CLL CD4+CD25+Tregs的凋亡有关。 |
| 关 键 词： | 四嗪二甲酰胺 慢性B淋巴细胞白血病 CD4+ CD25+调节性T细胞 细胞凋亡 流式细胞术 |
| 收稿时间： | 2010-10-14 |
Effects of ZGDHu-1 on CD4+CD25+ Regulatory T cells in Patients with Chronic B Cell Lymphocytic Leukemia in vitro |
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| Qiu Liannü,Zhou Yonglie,Huang Qiang,Hu Weixiao. Effects of ZGDHu-1 on CD4+CD25+ Regulatory T cells in Patients with Chronic B Cell Lymphocytic Leukemia in vitro[J]. Journal of Medical Research, 2011, 40(3): 34-39 | |
| Authors: | Qiu Liannü Zhou Yonglie Huang Qiang Hu Weixiao |
| Affiliation: | .Central Laboratory Medician,Zhejiang Provincial pepole′s Hospital,Zhejiang 310014,China |
| Abstract: | Objective To investigate the effects of ZGDHu-1 on CD4+CD25+ regulatory T cells in patients with chronic B cell lymphocytic leukemia(B-CLL) to elucidate its effect mechanism on CD4+CD25+Tregs.Methods Lymphocytes were isolated from PMBCs in patients with B-CLL and cocultured with or without ZGDHu-1.CD4+CD25+/highCD127low,CD19+CD5+ and viability rate,apoptosis,Fas,FasL,APO2.7,Bcl-2 and Bax of CD4+CD25+Tregs were detected with flow cytometry,respectively.Results CD4+CD25+/highCD127low,CD19+CD5+ in B-CLL were s... |
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