| Biological Characteristics of Human Bone Marrow Mesenchymal Stem Cell Cultured in Vitro |
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| 作者姓名： | 法宪恩 王利霞 侯剑峰 张瑞成 王海永 杨辰垣 |
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| 作者单位： | [1]DepartmentofCardiacSurgery,TheSecondaryHospitalofZhengzhouUniversity,Zhengzhou450003，China [3]DepartmentofCardiacSurgery,UnionHospital,TongjiMedicalCollage,HuazhongUniversityofScienceandTechnology,Wuhan430022,China |
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| 摘 要： | Some biological characteristics of human bone marrow mesenchymal stem cells (MSCs) cultured in vitro were observed, hMSCs were isolated from bone marrow and purified by density gradient eentrifugation method, and then cultured in vitro. The proliferation and growth characteristics of hMSCs were observed in primary and passage culture. MSCs of passage 3 were examined for the purify by positive rate of CD29 and CD44 through flow eytometry. Human bone marrow MSCs showed active proliferation capacity in vitro. The purify of MSCs separated by our method was higher than 90%. It was concluded that hMSCs have been successfully cultured and expanded effectively. It provided a foundation for further investigation and application of MSCs。
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| 关 键 词： | 生物学特征 骨髓间叶干细胞 细胞移植 细胞修饰 |
Biological Characteristics of Human Bone Marrow Mesenchymal Stem Cell Cultured in Vitro |
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| FA Xian'en,WANG Lixia,Hou Jianfeng,ZHANG Ruicheng,Wang Haiyong,YANG Chenyuan.Biological Characteristics of Human Bone Marrow Mesenchymal Stem Cell Cultured in Vitro[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2005,25(3):307-309. |
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| Authors: | FA Xian'en WANG Lixia Hou Jianfeng ZHANG Ruicheng Wang Haiyong YANG Chenyuan |
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| Abstract: | Some biological characteristics of human bone marrow mesenchymal stem cells (MSCs) cultured in vitro were observed. hMSCs were isolated from bone marrow and purified by density gradient centrifugation method, and then cultured in vitro. The proliferation and growth characteristics of hMSCs were observed in primary and passage culture. MSCs of passage 3 were examined for the purify by positive rate of CD29 and CD44 through flow cytometry. Human bone marrow MSCs showed active proliferation capacity in vitro. The purify of MSCs separated by our method was higher than 90 %. It was concluded that hMSCs have been successfully cultured and expanded effectively. It provided a foundation for further investigation and application of MSCs. |
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| Keywords: | mesenchymal stem cell bone marrow cell culture |
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