灵芝孢子粉中多糖的制备工艺研究

目的：针对灵芝孢子粉多糖的制备工艺进行研究。方法：以多糖转移率为指标，比较布袋包煎和搅拌提取两种工艺；采用单因素法，考察搅拌提取中料液比和提取次数对多糖提取的影响；根据滤液的性状，比较静置取上清液、离心、布袋滤过和板框滤过等多种滤过方法，优化滤过工艺。结果：灵芝孢子粉多糖的最佳制备工艺条件为：按料液比1：30加水至提取罐中，煮至100℃，投料，保温搅拌提取2min，静置24min，取上清液，离心，滤液先通过（5μm滤袋），再板框滤过，滤液浓缩至相对密度为1．10（60℃），醇沉，取沉淀减压干燥，千膏得率为3．26％，多糖含量为25．1％，多糖转移率97％。结论：该工艺可行有效，具有实际生产意义。

Study on the Preparation Technology of Polysaccharide of Ganoderma Lucidum Spore

Objective： To study the preparation technology of polysaccharide extracted from ganoderma lucidum spore. Methods： The transfer rate of polysaccharide was used as an index to compare the processes of cloth bag decoction and stirring extractions. By the single factor method, the effect of extraction times and the ratio of water to material on the yield of polysaccharide extracted from spores of granoderma atrum were investigated. According to properties of filtrate the preparation methods were studied including static supernatant extraction, centrifugation, bag filtration and plate and frame filter so as to optimize the filtration technology. Results： The optimized preparation conditions for polysaccharide of ganoderma lucidum spore were as follows：add water to the extraction pot according to the material liquid ratio 1：30, heating to 100 ℃ , then adding the material, stirring with heat preservation and extraction for 2 min, standing for 24 min, collect the the supernatant, centrifuge, filtrate through （5 μm bag） and plate and frame filter, concentrate the filtrate to a relative density of 1.1O （60 ℃ ）, alcohol precipitation, collect the precipitate and vacuum drying, the dry extract rate was 3.26%, polysaccharide content was 25.1%, and transfer rate of polysaccharide was 97%. Conclusion： The process is feasible and effective and has practical significance to production.