蝙蝠葛碱对实验小鼠脑缺血的保护作用

目的　研究蝙蝠葛碱 (dauricine,Dau)对小鼠缺氧及急性脑缺血再灌注所致氧化损伤及能量代谢障碍的保护作用。方法　通过常压密闭缺氧实验观察Dau对小鼠缺氧情况下存活时间的影响 ;采用酶标仪及分光光度法测定小鼠急性脑缺血再灌注后脑皮层组织和线粒体氧化损伤指标及能量代谢指标活性或含量变化。结果　①Dau延长小鼠缺氧后存活时间。②Dau改善脑缺血再灌注损伤所致脑皮层组织及线粒体SOD活性下降、MDA含量升高及线粒体GSH Px活性下降 ;改善脑皮层组织LDH及线粒体ATPase活性下降。结论　①Dau对小鼠急性缺氧具有保护作用。②Dau对脑缺血再灌注所致脑皮层组织及神经细胞线粒体氧化损伤及能量代谢障碍具保护作用

The protective effect of dauricine on cerebral ischemia in mice

AIM To investigate the protective effects of dauricine on anoxia and acute cerebral ischemia-reperfusion in mice. METHODS In this study, anoxia was induced by close normobaric hypoxia to search the effects of dauricine on the survival time of mice. The activities of SOD, GSH-Px and levels of MDA in cortex or mitochondria, the activities of cortex LDH and mitochondria ATPase were all measured by ELISA-Reader and spectrophotography. RESULTS ① Dauricine prolonged the survival time of mice in the close normobaric hypoxia, among all doses, Dauricine 60 mg·kg -1 and 120 mg·kg -1 had significant most effects (P<0.05). ② The decreased activities of total SOD were both enhanced in cortex and mitochondria by dauricine(in 30, 60, 120 mg·kg -1), the same effect on GSH-Px only appeared in mitochondria. The increased levels of MDA in cortex and mitochondria were all decreased by dauricine. ③ Dauricine(in 30, 60, 120 mg·kg -1) enhanced the decreased activities of cortex LDH and mitochondria ATPase. CONCLUSION ① Dauricine had protective effect on mice acute anoxia. ② Dauricine prevented and reversed the oxidative damage on cortex and mitochondria of brain induced by repeatedly cerebral ischemia reperfusion. ③ Dauricine ameliorated energy metabolism disturbance of cortex and mitochondria caused by repeatedly cerebral ischemia reperfusion.