流式细胞术检测甲状腺癌针吸组织中T淋巴细胞亚群及吞噬细胞糖蛋白的临床意义

探讨甲状腺癌组织中Ｔ淋巴细胞亚群（ＣＤ３、ＣＤ４、ＣＤ８）及吞噬细胞糖蛋白（ＣＤ４４）表达的规律，并研究免疫状态与肿瘤转移的关系，通过流式细胞免疫法对１３例甲状腺癌细针穿刺标本进行测定，并与甲状腺良性肿块对照。结果表明：甲状腺癌组ＣＤ３、ＣＤ４、ＣＤ８及ＣＤ４４阳性细胞较良性组均有明显变化，其中ＣＤ３、ＣＤ４阳性细胞明显下降（Ｐ＜０．０１），ＣＤ８阳性细胞明显增加（Ｐ＜０．０１），ＣＤ４／ＣＤ８比值明显降低（Ｐ＜０．０１），有转移者其变化更明显。ＣＤ４４阳性细胞在甲状腺癌组明显高于良性肿块组（Ｐ＜０．０１），且与转移有关。提示Ｔ淋巴细胞亚群紊乱使肿瘤细胞摆脱免疫监视而易于转移。本法操作简便，术前进行检查，可对患者免疫状态、肿瘤诊断、病程进展的估计及术前决策有帮助。

DETECTATION OF CD 3,CD 4,CD 8 AND CD 44 CONTENT OF FINE NEEDLE ASPIRATION SPECIMENTS IN THYROID CANCER PATIENTS

To search for the relationship between immune state and tumor metastases, CD 3,CD 4,CD 8 and CD 44 contents in 13 speciments of fine needle aspiration (FNA) from thyroid cancer patients were detected by the flow cytometry (FCM) and comparison between thyroid cancer and benign tumor was made. The result showed :in thyroid cancer group, CD 3,CD 4 cells and the ratio of CD 4/CD 8 reduced significantly ( P <0.01),and CD 8 cell increased significantly ( P <0.01), in metastases group,this change was much significantly. CD 44 expressed significantly higher in cancer group than that of the benign thyroid neoplasms, and the change was related to the tumor metastases. The results indicate that CD 44 can be as a marker of tumor and indicator of metastases. The disturbance of immune system results in active expression of CD 44 by tumor cells, so, lead to metastases. It is helpful to the diagnosis of thyroid cancer, assessment of metastases and management in surgery.