| 膜联蛋白5在大鼠消化器官中的细胞定位 |
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| 引用本文： | 张艳梅,韩雪峰,卢坤刚,陶晓倩,柳海燕,时姗姗,姚兵. 膜联蛋白5在大鼠消化器官中的细胞定位[J]. 医学争鸣, 2009, 30(9) |
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| 作者姓名： | 张艳梅 韩雪峰 卢坤刚 陶晓倩 柳海燕 时姗姗 姚兵 |
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| 作者单位： | 张艳梅,韩雪峰,卢坤刚,陶晓倩,柳海燕,时姗姗,ZHANG Yan-Mei,HAN Xue-Feng,LU Kun-Gang,TAO Xiao-Qian,LIU Hai-Yan,SHI Shan-Shan(南京师范大学生命科学学院,江苏,南京,210046);姚兵,YAO Bing(南京师范大学生命科学学院,江苏,南京,210046;南京军区南京总医院解放军临床检验医学研究所,江苏,南京,210002) |
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| 基金项目： | 国家自然科学基金，江苏省自然科学基金 |
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| 摘 要： | 目的:观察膜联蛋白5(Annexin 5)在大鼠部分消化器官中的细胞定位,为探讨Annexin 5在消化器官内可能发挥的生理学作用奠定基础.方法:选取健康SD大鼠,经Bouin's液固定其消化器官(胃、小肠、肝脏、胰腺)后,应用特异的兔抗Annexin 5抗血清,采用免疫组织化学方法和免疫荧光化学方法,观察Annexin 5在大鼠消化器官中的细胞定位.结果:Annexin 5在大鼠的消化器官中呈细胞特异性分布.Annexin 5在胃上皮细胞、胃底腺部分细胞、小肠绒毛杯状细胞、胆管内皮细胞和血管内皮细胞中分布较多,同时在胃和肠的黏膜层和肌层的部分细胞、小肠固有层中某些细胞、肝内皮细胞、胰腺血管内皮细胞中也有分布.结论:Annexin 5存在于大鼠消化器官执行特殊功能的一些细胞内,提示Annexin 5可能在这些细胞发挥生理功能的过程中具有特定的作用.
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| 关 键 词： | 膜联蛋白5 消化器官 定位 |
Cellular localization of annexin 5 in rat's digestive organs |
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| ZHANG Yan-Mei,HAN Xue-Feng,LU Kun-Gang,TAO Xiao-Qian,LIU Hai-Yan,SHI Shan-Shan,YAO Bing,College of Life Science,Nanjing Normal University,Nanjing ,China. Cellular localization of annexin 5 in rat's digestive organs[J]. Negative, 2009, 30(9) |
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| Authors: | ZHANG Yan-Mei HAN Xue-Feng LU Kun-Gang TAO Xiao-Qian LIU Hai-Yan SHI Shan-Shan YAO Bing College of Life Science Nanjing Normal University Nanjing China |
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| Affiliation: | ZHANG Yan-Mei1,HAN Xue-Feng1,LU Kun-Gang1,TAO Xiao-Qian1,LIU Hai-Yan1,SHI Shan-Shan1,YAO Bing1,21College of Life Science,Nanjing Normal University,Nanjing 210046,China,2Institute of Clinical Laboratory Medicine,Nanjing General Hospital,Nanjing Military Area Command,Nanjing 210002 |
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| Abstract: | AIM:To investigate the cellular distribution of annexin 5 in rat's digestive organs using immunohistochemistry and immunofluorescence. METHODS: Digestive organs from healthy rats were fixed with Bouin's solution. Annexin 5 was examined for its specific distribution in rat digestive organs by immunohistochemistry and immunofluorescence with specific antiserum to rat annexin 5. RESULTS: Annexin 5 was localized in digestive organs of rats in specific cell types. Annexin 5 mainly existed in epithelial cells in ... |
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| Keywords: | annexin 5 digestive organs localization |
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