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| 常用藏药“蒂达(藏茵陈)”各品种的薄层色谱鉴别研究 | |
| 引用本文： | 阳勇,钟国跃,武小赟,罗维早,谷丹丹. 常用藏药“蒂达(藏茵陈)”各品种的薄层色谱鉴别研究[J]. 中国中药杂志, 2013, 38(5): 757-761 |
| 作者姓名： | 阳勇 钟国跃 武小赟 罗维早 谷丹丹 |
| 作者单位： | 1. 重庆市中药研究院,重庆,400065 2. 重庆市中药研究院,重庆400065 ;江西中医学院,江西南昌330004 3. 重庆市中药研究院,重庆400065;成都中医药大学,四川成都611730 |
| 基金项目： | 国家科技支撑计划项目子课题(2006DA106A11-12);创新药物孵化基地项目子课题(2010ZX09401-306-3-9);国家科技重大专项子课题(2012ZX09103201-050) |
| 摘 要： | 目的:建立藏药“蒂达”不同药材品种的薄层色谱鉴别方法.方法:采用硅胶G板、三氯甲烷-甲醇(40∶1)展开,分离鉴别“蒂达”不同药材品种中的齐墩果酸成分.结果:对70个来源于龙胆科獐牙菜属、花锚属、扁蕾属、肋柱花属及虎耳草科虎耳草属的“蒂达”各品种药材中齐墩果酸的薄层色谱鉴别表明,除虎耳草外,其他来源品种中均检出有齐墩果酸,同种的各样品间薄层图谱特征一致,但同属不同种问及同科不同属间薄层图谱特征存在一定差异.结论:齐墩果酸的薄层鉴别方法可用于“藏茵陈”药材部分种的鉴别,可以有效鉴别来源于虎耳草科与其他种的“蒂达”样品. |
| 关 键 词： | 藏茵陈 薄层色谱鉴别 齐墩果酸 |
| 收稿时间： | 2012-08-08 |
Study on TLC identification of Dida commonly used in Tibetan medicine from different species |
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| YANG Yong,ZHONG Guo-yue,WU Xiao-yun,LUO Wei-zao and GU Dan-dan. Study on TLC identification of Dida commonly used in Tibetan medicine from different species[J]. China Journal of Chinese Materia Medica, 2013, 38(5): 757-761 | |
| Authors: | YANG Yong ZHONG Guo-yue WU Xiao-yun LUO Wei-zao GU Dan-dan |
| Affiliation: | Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China;Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China;Jiangxi University of Traditional Chinese Medicine, Jiangxi 330004, China;Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China;Chengdu University of Traditional Chinese Medicine, Chengdu 611730, China;Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China;Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China;Chengdu University of Traditional Chinese Medicine, Chengdu 611730, China |
| Abstract: | Objective: To establish a method of TLC identification for Dida commonly used in Tibetan medicine from different species. Method: With silica gel G as the stationary phase, and chloroform-methanol (40:1) as mobile phase, oleanolic acid from different species of Dida was separated and identified. Result: Oleanolic acid was detected in 70 kinds of Dida derived from the Gentianaceae Swertia, Halenia, Gentianopsis, Lomatogonium, and Saxifragaceae saxifrage, except for the saxifrage, there are some differences among different genera or subjection. Conclusion: This TLC method can be used for identification of oleanolic acid in Dida from different species except saxifrage. |
| Keywords: | Dida TLC oleanolic acid |
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