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| 多指标正交试验优化菊花提取工艺 | |
| 引用本文： | 张星海,李嬛,秦昆明,刘晓,汪斌,蔡宝昌.多指标正交试验优化菊花提取工艺[J].中国中药杂志,2013,38(6):821-824. |
| 作者姓名： | 张星海 李嬛 秦昆明 刘晓 汪斌 蔡宝昌 |
| 作者单位： | 1. 南京中医药大学国家教育部中药炮制规范化及标准化工程研究中心,江苏南京,210029 2. 南京中医药大学国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210029;南京海昌中药集团有限公司,江苏南京210061 3. 南京中医药大学国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210029;南京海昌中药集团有限公司,江苏南京210061;国家中医药管理局中药炮制标准重点研究室,江苏南京210046 4. 南京海昌中药集团有限公司,江苏南京,210061 |
| 基金项目： | 国家科技部火炬计划项目(2010GH020663);南京市科技发展计划项目(201007005);江苏省企业博士集聚计划项目 |
| 摘 要： | 目的:通过正交试验设计,探索量化的菊花评价指标,对菊花的提取工艺进行优化.方法:选择乙醇浓度、乙醇倍数、提取时间、提取次数4个因素,采用L9(34)安排试验,以菊花指纹图谱中8个主要共有峰的峰面积和浸出物含量作为指标,进行综合评价.结果:优选出菊花最佳提取工艺,采用超声波提取法,加入30倍80％乙醇溶液,提取40 min,共2次.结论:优化的提取工艺结果可靠,评价指标可控,对于规范菊花的提取工艺和菊花饮片的质量控制具有一定的意义. |
| 关 键 词： | 菊花 正交试验 指纹图谱 多指标 提取工艺 |
| 收稿时间： | 2012/10/27 0:00:00 |
Optimization of extraction process of Chrysanthemi Flos by multi-index orthogonal experiment |
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| ZHANG Xing-hai,LI Huan,QIN Kun-ming,LIU Xiao,WANG Bin and CAI Bao-chang.Optimization of extraction process of Chrysanthemi Flos by multi-index orthogonal experiment[J].China Journal of Chinese Materia Medica,2013,38(6):821-824. | |
| Authors: | ZHANG Xing-hai LI Huan QIN Kun-ming LIU Xiao WANG Bin and CAI Bao-chang |
| Institution: | Engineering Research Center for Processing Standardization of Traditional Chinese Medicine under Ministry of Education, Nanjing University of Chinese Medicine, Nanjing 210029, China;Engineering Research Center for Processing Standardization of Traditional Chinese Medicine under Ministry of Education, Nanjing University of Chinese Medicine, Nanjing 210029, China;Nanjing Haichang Chinese Medicine Group Co., Ltd., Nanjing 210061, China;Engineering Research Center for Processing Standardization of Traditional Chinese Medicine under Ministry of Education, Nanjing University of Chinese Medicine, Nanjing 210029, China;Nanjing Haichang Chinese Medicine Group Co., Ltd., Nanjing 210061, China;Key Laboratory of Processing Standardization of Traditional Chinese Medicine State Administration of Traditional Chinese Medicine, Nanjing 210046, China;Engineering Research Center for Processing Standardization of Traditional Chinese Medicine under Ministry of Education, Nanjing University of Chinese Medicine, Nanjing 210029, China;Nanjing Haichang Chinese Medicine Group Co., Ltd., Nanjing 210061, China;Engineering Research Center for Processing Standardization of Traditional Chinese Medicine under Ministry of Education, Nanjing University of Chinese Medicine, Nanjing 210029, China;Nanjing Haichang Chinese Medicine Group Co., Ltd., Nanjing 210061, China;Key Laboratory of Processing Standardization of Traditional Chinese Medicine State Administration of Traditional Chinese Medicine, Nanjing 210046, China |
| Abstract: | Objective: To explore quantitative assessment indicators of Chrysanthemi Flos, and optimize the extraction process of Chrysanthemi Flos through orthogonal experimental design. Method: The concentration of ethanol, amount of ethanol, extraction time and extraction frequency were selected as factors in the L9(34) orthogonal experiment. A comprehensive assessment was conducted with the peak area of the eight major common peaks in the fingerprint of Chrysanthemi Flos as the indicators. Result: The optimum extraction process was selected as follows: using ultrasonic extraction method, adding 30-fold ethanol with 80% concentration, extracting for 2 times for extraction, 40 min for each time. Conclusion: The optimized extraction process is reliable, with controllable assessment indicators, which is significant to the standardization of the extraction process and quality control of Chrysanthemi Flos preparations. |
| Keywords: | Chrysanthemi Flos orthogonal experiment fingerprint multi-index extraction process |
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