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| 高表达microRNA-22对缺氧心肌细胞的保护作用及机制 | |
| 引用本文： | 凌琳,凌子成,顾少华. 高表达microRNA-22对缺氧心肌细胞的保护作用及机制[J]. 中国动脉硬化杂志, 2017, 25(10): 997-1001 |
| 作者姓名： | 凌琳 凌子成 顾少华 |
| 作者单位： | 苏州大学附属第一医院心血管内科,江苏省苏州市 215006,扬州大学医学院,江苏省扬州市 225000,昆山市第三人民医院,江苏省昆山市215300 |
| 基金项目： | 江苏省自然科学青年基金项目(BK20140296)；苏州市科教兴卫项目(KJXW2013004)；苏州大学校级青年教师自然科学基金项目(SDY2013A29) |
| 摘 要： | 目的观察高表达microRNA-22对体外培养的缺氧心肌细胞的保护作用及机制。方法用携带microRNA-22的腺病毒载体和空载体转染体外缺氧条件下培养的原代心肌细胞,检测高表达microRNA-22后心肌细胞生物学特性的改变。采用MTT检测细胞活力,EdU检测细胞DNA合成能力,Caspase-3检测细胞凋亡情况。结果表达microRNA-22的腺病毒载体成功转染原代心肌细胞,流式细胞仪检测绿色荧光蛋白阳性细胞比例95%,高表达microRNA-22从蛋白水平显著下调PTEN表达。与空载腺病毒组相比,高表达microRNA-22组细胞核增殖明显增加,细胞生长更快,细胞活力增加,细胞凋亡减少。结论体外高表达microRNA-22能保护缺氧条件下的心肌细胞。 |
| 关 键 词： | microRNA-22 心肌细胞 细胞特性 |
| 收稿时间： | 2017-02-17 |
| 修稿时间： | 2017-06-25 |
Over-expression of microRNA-22 protects cardiomyocytes from ischemic injury |
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| LING Lin,LING Zi-Cheng and GU Shao-Hua. Over-expression of microRNA-22 protects cardiomyocytes from ischemic injury[J]. Chinese Journal of Arteriosclerosis, 2017, 25(10): 997-1001 | |
| Authors: | LING Lin LING Zi-Cheng GU Shao-Hua |
| Affiliation: | Department of Cardiology, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China,Medical School of Yangzhou University, Yangzhou, Jiangsu 225000, China and The Third People'' s Hospital of Kunshan, Kunshan, Jiangsu 215300, China |
| Abstract: | Aim To investigate the protection effect of microRNA-22 on ischemic cardiomyocyte. MethodsCardiomyocytes were transfected with microRNA-22 and cell activities were assessed. MTT was used to assess cell activities, EdU to assess DNA proliferation and Caspase-3 to assess cell apoptosis. Results MicroRNA-22 was transducted successfully using adeno-virus. The transduction efficiency reached 95%. Over-expression of microRNA-22 down-regulated PTEN level. Over-expression of microRNA-22 increased cell activities, DNA proliferation and decreased cell apoptosis. Conclusion Over-expression of microRNA-22 protected cardiomyocytes from ischemic injury. |
| Keywords: | MicroRNA-22 Cardiomyocyte Cell activities |
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