SysmexXE-5000血液分析仪检测异型淋巴细胞的实验研究

目的对XE-5000血液分析仪提示的异型淋巴细胞比率与人工镜检、T淋巴细胞亚群检测结果比较，探讨XE-5000血液分析仪对异型淋巴细胞检出的意义。方法收集该院血细胞分析仪XE-5000测定静脉血中结果报警出现异型淋巴细胞和无报警出现异型淋巴细胞的患儿样本各110例进行人工涂片，瑞氏染色及显微镜复检，并采用流式细胞仪对部分外周血T淋巴细胞亚群进行检测。结果以镜检为判断标准，XE-5000血液分析仪对异型淋巴细胞报警的灵敏度为90．82％，特异度为82．79％，阳性预测值为80．91％，阴性预测值为91．82％，仪器法与镜检法检测异型淋巴细胞结果差异有统计学意义（P〈O．05）；报警组CD3、CD8、CD4、CD4／CD8水平，分别与无报警组比较差异有统计学意义（P〈0．05）。结论XE-5000全自动血液分析仪异型淋巴细胞报警信息作为血常规分析中出现异型淋巴细胞的参考，对临床诊断效率的提高有一定帮助。

Experimental study on detection of atypical lymphocyte by Sysmex XE -5000 hematology analyzer

Objective To analyze the significance of atypical lymphocyte detection by Sysmex XE-5000 hematology analyzer,and compare the results of manual microscopy and the expression level of T lymphocyte subsets. Methods 110 cases with atypical lym- phocyte that had abnormal prompt and 110 cases with no abnormal prompt were collected,and the results were compared with all cases of manual push chip and Wright stain microscopy. Then T lymphoctyte subsets in peripheral blood with part of abnormal alert were measured by no abnormal alert. Results Taking microscopy as the relative standard method,the alarm＇s ensitivity of atypical lymphocytes for Sysmex XE-5000 hematology analyzer was 90. 82% specificity was 82. 79% positive predictive value was 80.91%, and negative predictive value was 91.82 %. There were differences between hematology analyzer and manual microscopy in the percentage of atypical lymphocytes. Compared with no abnormal prompt, the leves of CD3, CD8, CD4,CD4/CD8 were signifi- cantly different in abnormal prompt. Conclusion Abnormal prompt of atypical lymphocyte/or XE-5000 hematology analyzer can be used as an effective reference tool,and XE-5000 can improve the efficiency of clinical diagnosis.