| 人可溶性TRAIL蛋白诱导多种肿瘤细胞的凋亡 |
| |
| 引用本文： | 郑晓勇,官孝群,林芷英.人可溶性TRAIL蛋白诱导多种肿瘤细胞的凋亡[J].中华医学杂志,2000,80(3):190-192. |
| |
| 作者姓名： | 郑晓勇 官孝群 林芷英 |
| |
| 作者单位： | 上海医科大学肝癌研究所(郑晓勇!200032,林芷英!200032,汤钊猷!200032),上海医科大学分子遗传研究室(官孝群,宋后燕) |
| |
| 基金项目： | 上海市卫生局医学领先专业科研基金资助项目(983001) |
| |
| 摘 要： | 探讨原核表达的凋亡激活因子－人可溶性ＴＲＡＩＬ蛋白放导多种肿瘤,尤其实体瘤细胞凋亡的人作用及与Ｐ５３突变的关系。方法ＰＣＲ扩增人ＴＲＡＩＬ密码子１１４－２８１,ＪＦ１１２５大肠杆菌表达,复发并纯化该可溶性片段。免疫组织化学分析各细胞株Ｐ５３的突变。
|
| 关 键 词： | 肿瘤 脱噬作用 p53 TRAIL蛋白 细胞凋亡 |
| 修稿时间： | 1999-02-24 |
Induction
of apoptosis in various cancer cell lines by human soluble TRAIL expressed in Escherichia
coli |
| |
| ZHENG Xiaoyong ,GUAN Xiaoqun,LIN Zhiying,et al..Induction
of apoptosis in various cancer cell lines by human soluble TRAIL expressed in Escherichia
coli[J].National Medical Journal of China,2000,80(3):190-192. |
| |
| Authors: | ZHENG Xiaoyong GUAN Xiaoqun LIN Zhiying |
| |
| Institution: | Liver Cancer Institute, Shanghai Medical University, Shanghai 200032, China. |
| |
| Abstract: | Objective To investigate the induction of
apoptosis by human soluble TRAIL expressed in Escherichia coli and analyze its
relationship with p53 in various cancer cell lines, especially the solid tumor. Methods TRAIL codons 114～281 were amplified by polymerase
chain reaction. The soluble segment was expressed in Escherichia coli JF1125, refolded and
purified. Induction of apoptosis was assessed by examination of morphological changes
under electron microscope and quantified by FACS analysis. The mutation of p53 in employed
cell lines was detected by immunohistochemistry. Results By multi-step purification, the
purity of human soluble TRAIL exceeded 90%. Induction of apoptosis by the product
exhibited a marked increase in human breast cancer cell line MCF-7, HeLa cervical
carcinoma cells and hepatocellular carcinoma cell lines SMMC7721, BEL7402, and BEL7405.
The mutation of p53 in employed cell lines was positive in aforementioned cell lines. Conclusion The human soluble TRAIL expressed
in Escherichia coli has similar activity to that generated in cells to induce apoptosis in
various cancer cell lines, and acts independently of p53. |
| |
| Keywords: | Cancer Apoptosis Gene p53 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
