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| 牡丹皮高效液相色谱指纹图谱研究 | |
| 引用本文： | 许舜军,李鹏,杨柳,张勉,王峥涛. 牡丹皮高效液相色谱指纹图谱研究[J]. 中国中药杂志, 2006, 31(20): 1677-1680 |
| 作者姓名： | 许舜军 李鹏 杨柳 张勉 王峥涛 |
| 作者单位： | 1. 中国药科大学,生药学研究室,江苏,南京,210038 2. 广州市邮电医院,广东,广州,510603 3. 广东省中医院,中心实验室,广东,广州,510120 |
| 基金项目： | 国家中医药管理局资助项目 |
| 摘 要： | 目的：建立牡丹皮的高效液相色谱指纹图谱,为科学评价及有效控制其质量提供可靠方法。方法：利用HPLC-DAD,梯度洗脱,测定了30批牡丹皮药材样品,并应用LC-MS和MS-MS技术指认指纹图谱中的主要色谱峰。结果：30批牡丹皮样品得到的色谱指纹图谱有15个共有峰,指认了色谱峰的化学归属,主要特征峰的峰面积排序依次为：丹皮酚>五没食子酰葡萄糖>没食子酸甲酯>没食子酰芍药苷>没食子酸>羟基芍药苷>其他色谱峰。结论：牡丹皮的指纹图谱特征性及专属性强,可结合含量测定用于全面控制牡丹皮的质量。 |
| 关 键 词： | 牡丹皮 高效液相色谱 指纹图谱 |
| 文章编号： | 1001-5302（2006）20-1677-04 |
| 收稿时间： | 2005-12-02 |
| 修稿时间： | 2005-12-02 |
Studies on HPLC fingerprint of Cortex Moutan |
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| XU Shun-jun ;LI Peng ;YANG Liu ;ZHANG Mian ;WANG Zheng-tao. Studies on HPLC fingerprint of Cortex Moutan[J]. China Journal of Chinese Materia Medica, 2006, 31(20): 1677-1680 | |
| Authors: | XU Shun-jun LI Peng YANG Liu ZHANG Mian WANG Zheng-tao |
| Affiliation: | 1. Department of Pharmacognosy, China Pharmaceutical University, Nanfing 210038, China; 2. Guangzhou Post and Telegraph Hospital, Guangzhou 510630, China; 3. Second Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510120, China |
| Abstract: | Objective: To establish a HPLC fingerprint for quality evaluation of Cortex Moutan.Method: The HPLC chromatographic fingerprinting of 30 lots of Cortex Moutan was established and major peaks were identified by LC-MS and MS-MS.Result: The HPLC fingerprint of Cortex Moutan was established,showing 15 characteristic peaks.The areas of these peaks were found to complied with the following rule: paeonol > 1,2,3,4,6-penta-O-galloyl-glucos > methyl gallate > galloylpaeoniflorin > gallic acid > oxypaeoniflorin > other compounds.Conclusion: The chromatographic fingerprinting of Cortex Moutan with high specificity can be used to control its quality and monitor lot to lot consistency. |
| Keywords: | Cortex Moutan HPLC fingerprinting |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |