献血者乙型肝炎病毒体液免疫和细胞免疫水平研究

目的:研究自然状态下献血者对乙型病毒性肝炎不同抗原成份的体液、细胞免疫应答水平,及两者的相关性。方法:ELISA检测献血者血浆中HBsAb、HBcAb、preS1+S2-Ab的阳性率,ELISOPT检测外周血单个核细胞中抗原特异性IFN-γ分泌细胞水平。结果:体液免疫应答检测结果表明preS1+S2-Ab的检出率最高,占85.7%(48/56),核心杭体的检出率最低30.4%(17/56);特异性细胞免疫检测结果提示献血者总体preS1+S2-Ag、HBcAg的特异性细胞免疫应答水平有着良好的一致性,无统计学差异(P>0.05),但显著优于HBsAg;preS1+S2-Ag和HBsAg特异性细胞免疫和体液免疫应答有着较好的一致性,但HBcAg特异性细胞免疫和体液免疫无明确关联(P>0.05)。结论:自然状态下献血者对乙型病毒性肝炎不同抗原成份均有较好的体液、细胞免疫应答,preS1+S2的体液免疫应答水平优于表面及核心抗原提示人HBV疫苗的侯选对象。

Humoral and cellular immune responses to HBV in the blood donors

AIM: To study the magnitude and correlation of humoral and cellular immune responses to hepatitis surface antigen(HBsAg),preS1+S2 antigen,and core antigen(HBcAg) in the blood donors.METHODS: Enzyme linked immunosorbent assay(ELISA) was employed to scan the humoral immune responses to HBsAg,preS1+S2 antigen,and HBcAg.Enzyme linked immunospot assay(ELISPOT) was used to check the antigen specific IFN-γ secreting cells stimulated by HBsAg,preS1+S2 antigen or HBcAg in peripheral blood mononuclear cell from blood donors.RESULTS: The prevalence of humoral immune response to preS1+S2 antigen was as high as 85.7%(48/56),compared with the lower response to HBcAg(30.4%,17/56).In the cellular immune response,there was no difference between preS1+S2 antigen and HBcAg,both of which were stronger than the response stimulated by HBsAg.PreS1+S2 antigen or HBsAg specific humoral immune and cellular immune responses were highly correlated,which could not be observed in the HBcAg specific humoral immune and cellular immune responses(P>0.05).CONCLUSION: Either humoral or cellular immune responses to HBsAg,preS1+S2 antigen and HBcAg could be detected in the blood donors.PreS1+S2 antigen immune responses,however,were most powerful,which may suggest that PreS1+S2 antigen may be a candidate for the further HBV vaccine.