孟加拉红荧光法辅助诊断口腔癌癌前病变的实验研究

目的 应用动物模型探讨孟加拉红(RB)介导的荧光光谱诊断法辅助诊断各期口腔癌癌前病变的可行性。方法 在405 nm的激发光下测定6种不同浓度RB溶液的光谱,并分析荧光强度与浓度之间的关系。20只金黄地鼠随机分成4组(对照组、4周、6周、8周组),每组5只。各组实验动物双侧颊囊经DMBA诱导相应时间后经0.1 g/L RB溶液染色5 min,应用光谱诊断装置分别记录自体及RB激发荧光并应用Origin 7.0软件计算荧光比值K值。检测位点经病理切片定性后计算各组K值均值,SPSS 10.0进行统计分析。结果 随着RB溶液浓度的提高,荧光强度亦增强,二者呈正态线性关系。动物实验结果经方差分析,4组K值均值相比有统计学意义(P<0.01)。结论 RB介导的荧光光谱法能够区分正常、单纯增生、轻度异常增生和重度异常增生的组织,对口腔癌癌前病变的诊断具有重要的参考价值。

A pilot study of Rose Bengal fluorometric method in auxiliary diagnosis of oral precancerosis

Objective To investigate the feasibility of fluorescence spectra diagnose mediated with Rose Bengal(RB) at different stages of oral precancerous lesions in animal models.Methods Excitated by 405 nm light,fluorescence spectrum of 6 different concentrations of RB solutions were detected under 405 nm light.The relation between fluorescence intensity and concentration was analyzed.20 golden hamsters were randomly divided into 4 groups(control group,4 weeks group,6 weeks group,and 8 weeks group),each group havin...