用单克隆抗体CL—4从粪便中检测大肠癌相关抗原的临床意义

Detection of Cancer-associated Antigens in Feces of Patients with Colorectal Cancer with Monoclonal Antibody CL-4

The level of CL-4 related antigens In feces was detected by binding inhibition SABC-ELISA method. It revealed that the mean inhibtion rate is significantly higher in colorectal cancer than that of non-malignant GI lesions(22%), and normal individual, 7.4%. In 12 out of 13 patients with colorectal cancer, it also showed that the antigen expression in tissues parallels to the level of antigen detected in the feces. In six out of eight patients, the level of antigen decreased after the resection of cancer, while the level of antigen of other two patients, one with liver metastasis, another with evidence of antigen involvement at the resected margin, still remained in their feces. No correlation was seen between the Dukes staging and the level of antigen. But the positive rate is higher in DukesA patients (5/6) than that of stage B, C, D. It is therefore suggested that useing of monoclonal antibody CL-4 to detect the related antigen directly excreted into the feces is superior to the detection in serum. It may provide a useful tool for diagnosis and monitor the colorectal cancer.