中国儿童急性淋巴细胞性白血病6q21区域微缺失的研究

目的:分析染色体6q2 1的D6S4 4 9 D6S30 2区域等位基因杂合性缺失的情况,确定其在中国儿童急性淋巴细胞性白血病中发生的频率和最小缺失区域,为定位克隆ALL相关抑癌基因提供线索。方法:采用比以往报道更高密度的11个微卫星多态性标记位点,利用聚合酶链反应 变性凝胶电泳银染技术分析93例儿童急性淋巴细胞性白血病例等位基因LOH的情况。结果:93例患者中共有32例(34.4 % )存在至少一个位点的LOH。位点D6S170 9 D6S30 1以及位点D6S30 2是高频率缺失集中的区域,位于其中的D6S2 83位点附近是缺失率最高的地方(2 5 % )。D6S170 9 D6S30 1之间距离在GenethonMap上显示有2cM。结论:6S170 9 D6S30 1为本研究所找到的6q2 1上的最小缺失区域,当中可能存在与中国儿童急性淋巴细胞性白血病发病相关的一个或一个以上的候选抑癌基因

Allelic Loss Mapping on 6q21 in Acute Lymphoblastic Leukemia of Children in China

Objective To analysis loss of heterozygosity (LOH) of D6 S449-D6S302 o n chromosome 6q21 and definite their occurrence and the minimus deletion regions in acute lymphoblastic leukemia of children in China, which was conducted to lo cate and find tumor suppressor genes correlated with ALL. Methods 11 locus on 6q21 in 93 illness were studied by using microsatellitoanalysis. Result s The f requency of LOH was 34.4%. The most commom LOH was identified at the loci of D6S 1709-D6S301, with a highest one around D6S283. The distance between D6S1709-D6 S3 01 is about 2 cM at GenethonMap. Conclusion The minimus de letion regions is f ound on 6q21, and there may be tumor suppressor gene condidates around D6S1709- D6S301.