广藿香对肢体缺血-再灌注大鼠肠上皮细胞膜流动性的保护作用

目的 观察广藿香对肢体缺血－再灌注大鼠血清一氧化氮（NO），肿瘤坏死因子（TNF α）及肠上皮细胞膜流动性的影响，探讨广藿香保护肠屏障功能的作用及机制。为从中医药角度寻找创伤、手术后等胃肠道保护措施新方法提供实验依据。

Protective Effect of Pogostemon cablin on Membrane Fluidity of lntestinal Epithelia Cell in lschemia/Reperfusion Rats after Ischemia/Reperfusion

Objective To observe the effects of Pogostemon cablin （Blanco） Benth. （ PCB）, a Chinese aromatic herbal medicine, on serum levels of nitric oxide （NO）, tumor necrosis factor-α （TNF-α）, and membrane fluidity of intestinal epithelial cells （IMC） in rats undergoing lower limbs ischemic reperfusion （ I/R）, for exploring its action in protecting intestinal barrier and the possible mechanisms, and to seek a new way, viewing from Chinese medicine, for providing the experimental bases of gastrointestinal protection against trauma or surgical operation. Methods Eighty adult Wistar rats were induced into lower limb I/R model and randomized equally into the model group, the three PCB water extract groups treated respectively with high- （4 g·kg^-1·d^-1 ）, middle- （3 g·kg^-1·d^-1）, and low-dose （2 g·kg^-1·d^-1 ） of PCB water extract, and three PCB volatile oil groups treated respectively with high- （4 g·kg^-1·d^-1）, middle- （3 g·kg^-1·d^-1）, low-dose （2 g·kg^-1·d^-1） of PCB volatile oil. Besides, 10 healthy rats was allocated in a normal control group. PCB preparation was given via gastric infusion for 5 successive days just before modeling. The serum levels of NO and TNF-α were monitored, and the membranous fluidity of IMC at I/R region was determined by fluorescence polarization technique. Results Compared with the control group, both serum NO and TNF-α levels in model rats were significantly higher （P〈0.01）, and the fluorescence polarization value （P） of IMC obviously increased at the same time （P 〈0.05）. As compared with the model group, the serum level of NO and TNF-α significantly reduced in all the PCB treated groups （ P 〈 0.01 or P 〈 0.05）. As for the membrane fluidity, significant difference was shown between the model group with low-dose of PCB water extract and middle-dose of PCB volatile oil （ P 〈0. 05）. Conclusion PCB could effectively protect the intestinal barrier function by way of maintaining the membrane fluidity of IMC through regulating the level of NO and TNF-α in serum.