毛冬青甲素对阿尔茨海默病模型大鼠学习记忆及海马神经元再生的影响

目的 探讨毛冬青甲素对阿尔茨海默病(Alzheimer's disease,AD)模型大鼠学习记忆能力及神经元再生的影响.方法 选取雄性SD大鼠24只,以Aβ1-42海马注射制作AD大鼠模型,随机分为4组,分别为假手术组、模型组、毛冬青甲素组、阳性药物组(多奈哌齐).于造模后第3天起开始干预,连续4周.干预结束后进行Morris水迷宫测试,记录各组大鼠水迷宫测试期间逃避潜伏期和空间探索实验中大鼠处于平台所在区间时间比率;用Brdu标记增殖细胞,并采用免疫组织化学法检测各组大鼠海马组织被标记的增殖细胞表达情况,甲苯胺蓝染色法观察海马组织锥体细胞Nissl体形态.结果 Morris水迷宫实验结果表明,与模型组相比,多奈哌齐组、毛冬青甲素组均能缩短潜伏期时间和增加穿越平台次数,改善其学习记忆能力,差异均有统计学意义(P<0.05);Brdu标记结果显示毛冬青甲素能改善海马齿状回区神经细胞再生.结论 毛冬青甲素能促进AD模型大鼠神经元再生,且显著改善AD大鼠空间学习记忆能力.

Effects of IlexoninA on Learning-Memory and Neuron Regeneration in the Hippocampal Neuron of Alzheimer's Disease Model Rats

Objective To investigate the effects of Ilexonin A on neurological behavior and neuron regeneration in Alzheimer's disease (AD) model rats. Methods The 24 male SD rats were randomly divided into sham -opera-tion group, model group, Ilexonin A group, positive medicine group (donepezil). Aβ1-42 was injected into hippocampus CA1 re-gion of male SD rats and to establish Alzheimer's disease-like model, then from the 3th day after modeling, the Ilexonin A or Donepezil drug gavage lasted 4 weeks. The learning and memory abilities of the rats were assessed through Morris water maze behavioral test, and neuron proliferation was tested by Brdu label. The expression of proliferative cells in hippocampus tissues was determined by immunohitochemical method. The morphology of hippocampal pyramidal neuron were observed by Nissl stain-ing. Results Compared with the model group, the learning and memory of the rats in the drug treated groups sgnificantly in-creased (P<0.05) and the levels of neuron proliferation were increased in the hippocampal dentate gyrus (DG) region of Ilexonin A treated rats. Conclusion The Ilexonin A reatment can promote neuron regeneration in the hippocampal DG region, and im-prove the learning and memory ability of the Alzheimer's disease-like model rats.