原花青素促进三阴乳腺癌MDA-MB-231细胞凋亡的作用机制研究

目的 研究原花青素(Procyanidins,PC)对三阴乳腺癌MDA-MB-231细胞株增殖及细胞凋亡的作用及其机制.方法 常规培养细胞,24 h后随机分为阳性对照组、 对照组和PC10、20、40、80、160、320μg/mL组.MTT法检测细胞生长抑制率;流式细胞术检测细胞凋亡;Western blot法检测FoxA1蛋白及抗凋亡蛋白bcl2、UCP2表达.结果 PC各剂量组均可显著抑制抑制MDA-MB-231细胞增殖(P<0.05);PC(10-160μg/mL)组剂量依赖性抑制MDA-MB-231细胞增殖(P<0.05),PC(40-320μg/mL)可以时间依赖性抑制细胞增殖(P<0.05);PC320μg/mL组各时间点细胞抑制率与PC160μg/mL组差异无显著性(P>0.05);160μg/mL PC可以时间依赖性促进MDA-MB-231细胞凋亡(P<0.05);160μg/mL PC作用24 h后,FoxA1蛋白表达显著上升(P<0.05),同时bcl2及UCP2表达降低(P<0.05).结论 PC抑制MDA-MB-231细胞,促进MDA-MB-231细胞凋亡,升高其FoxA1表达,同时降低bcl2及UCP2表达,表明PC可能通过促进FoxA1表达发挥促进MDA-MB-231细胞凋亡的作用.

Effect of Procyanidin in Promoting Apoptosis of MDA-MB-231 Triple-Negative Breast Cancer Cell Lines

Objective To study the effect and mechanism of procyanidins (PC) on the proliferation and apoptosis of MDA-MB-231 breast cancer cell line. Methods The cells were randomly divided into positive control group, control group and PC (10, 20, 4., 80, 160, 320 μg/mL) groups. Growth inhibiting rate of MDA-MB-231 cell was detected by MTT assay. Apoptosis was detected by flow cytometry, and the expression of FoxA1 protein and anti apoptotic protein (UCP2 and bcl2) were detected by Western blot. Results Different doses of PC could infinicantly inhibited the proliferation of MDA-MB-231 cells (P<0.05). The inhibition rate of cell growth in the range of 10-160 μg/mL was dose-dependent (P<0.05), and the inhibition rate of cell growth in the range of 40-320 μg/mL was time-dependent (P<0.05). However, there was no significant between 160 μg/mL group and 320μg/mL group in the inhibition rate of cell growth (P>0.05). 160 μg/mL PC could promote the apoptosis of MDA-MB-231 cells in the time-dependent (P<0.05). The expression of FoxA1 protein significantly increased after 160 g/mL PC for 24 h (P<0.05), while the expression of UCP2 and bcl2 decreased (P<0.05). Conclusion PC could promote the apoptosis of MDA-MB-231 cells and decrease the expression of bc12 and UCP2, which may through promoting the expression of FoxA1.