VEGF-C与鼻咽癌增殖和转移的关系

目的 检测血管内皮生长因子C（VEGF-C）在鼻咽癌（NPC）组织中的表达,探讨其与鼻咽癌细胞增殖和转移的关系。方法NPC患者放疗前的活检组织标本62例,将每例标本均分2份,一份应用VEGF-C多克隆抗体进行SP法免疫组化染色,另一份应用流式细胞术以Ki67抗体检测其增殖状况。放疗后定期随访患者,统计分析其3年生存率及死亡原因。结果62例NPC标本中,VEGF-C表达范围为0～82％,其中阴性（-）17例（27．4％）,弱阳性（＋）13例（21．0％）,中等阳性（＋＋）18例（29．0％）,强阳性（＋＋＋）14例（22．6％）。Ki67表达范围为0-52％,其中低表达（LPI）者40例（64．5％,含15例阴性）,高表达（HPI）者22例（35．5％）。VEGF-C与Ki67表达之间存在明显的正相关（r=0．323,P〈0．05）。3年生存率为66．1％。有淋巴结转移者的VEGF-C表达显著强于无淋巴结转移者（P〈0．01）。8例死于远处转移的患者中,VEGF-C表达均为强阳性;21例无病生存者中,VEGF-C表达为（-）或（＋）者占80．9％。VEGF-C表达与患者预后存在一定的负相关,但差异无统计学意义（r=-0．219,P〉0．05）。结论VEGF-C高表达与鼻咽癌细胞的增殖和转移能力相关,但不是影响患者预后的独立因素,而是一个预测无病生存期长短的指标。

Relationship between VEGF-C expression and nasopharyngeal carcinoma proliferation and metastasis

Objective To detect the expression of VEGF-C in nasopharyngeal carcinoma (NPC) and explore its relationship with proliferation and metastasis of NPC. Methods Biopsy specimens of 62 NPC patients were divided into 2 equal portions, one for immunohistochemical staining with VEGF-C polyclonal antibody by streptavidin peroxidase method, another for flow cytometry with Ki67 antibody to analyze the proliferation of tumors. The patients were followed up periodically, and then their 3-year survival and the cause of death were statistically analyzed. Results Of the 62 patients, the percentage of VEGF-C positive cells in the tumors ranged from 0 -82% , 17 (27.4% ) were negative, 13 (21.0% ) weak positive, 18 (29. 0% ) moderate positive and 14(22.6% ) strong positive. Ki67 positive tumor cells ranged from 0 -52% , 40 cases (64.5% ) showed low expression which include 15 cases of negative, 22 (35.5% ) showed high expression. There was a direct relationship between the expression of VEGF-C and Ki67 (r =0. 323, P <0. 05). The 3-year survival rate of 62 patients was 66. 1%. The expression of VEGF-C in the patients with positive lymph node was much higher than that with negative lymph node ( P < 0. 01 ). Among the 8 patients with distant metastasis, their VEGF-C expression was strong positive while among the 21 disease free survival patients, their VEGF-C expression was ( - ) or ( + ) account for 80. 9%. An inverse correlation was found between the VEGF-C expression and prognosis of NPC patients, but the difference has no statistical significance (r= -0.219, P>0.05). Conclusion High expression of VEGF-C is related with proliferation and metastasis of NPC cells. It is not an independent prognostic factor, but a predictive marker of disease-free survival of NPC patients.