| Abstract: | ![]()
Calcium ionophore A23187 increases the rate of spontaneous catecholamine release from cat adrenal glands perfused at 37 degrees C with oxygenated Krebs bicarbonate solution, in a time- and Ca-concentration-dependent manner. The secretory profile obtained with the ionophore was not modified in the presence of the Ca channel activator Bay K 8644. Ouabain also enhanced the rate of spontaneous catecholamine outputs in a time- and concentration-dependent manner. The threshold ouabain concentration capable of producing a clear, yet delayed secretory response was 10(-6) M. Increasing ouabain concentrations up to 10(-4) M enhanced catecholamine release and shortened the time to peak release. The dihydropyridine Ca channel activator Bay K 8644 (10(-6) M) markedly potentiated the secretory effects of all ouabain concentrations used (10(-7)-10(-4) M). However, the most impressive potentiations were seen at 10(-5)M ouabain; while at this concentration ouabain alone released 2.6 +/- 0.07 micrograms catecholamines per 30 min, in the presence of Bay K 8644 the release was 73.4 +/- 5.7 micrograms per 30 min. Conversely, at a fixed ouabain concentration (10(-5) M), the potentiation was also dependent on the Bay K 8644 concentration (10(-8)-10(-5) M). Although K deprivation inhibits Na pumping as does ouabain, Bay K 8644 did not modify the rate of catecholamine release evoked by K removal from the perfusion medium. Potassium deletion, nimodipine or high Mg all reversed the fully developed secretory response evoked by ouabain plus Bay K 8644. In glands depolarized by continuous perfusion with high K solutions, once the secretory response was inactivated, the introduction of ouabain caused an enhancement of the catecholamine secretory rate.(ABSTRACT TRUNCATED AT 250 WORDS) |
