| C57小鼠源性淋巴瘤的基因枪途径抗肿瘤免疫研究 |
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| 引用本文： | 李瑞永,蔡春玉,金文昊,张叔人.C57小鼠源性淋巴瘤的基因枪途径抗肿瘤免疫研究[J].延边医学院学报,2005,28(1):16-19. |
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| 作者姓名： | 李瑞永 蔡春玉 金文昊 张叔人 |
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| 作者单位： | 延边大学医学院附属医院肿瘤科 吉林延吉133000
(李瑞永，金文昊)，延边大学医学院附属医院呼吸内科 吉林延吉133000
(蔡春玉)，中国医学科学院肿瘤研究所免疫室 北京100021(张叔人) |
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| 摘 要： | 目的]诱导C57小鼠建立有效的抗肿瘤免疫.方法]用基因枪途径给C57小鼠腹部接种含有粒巨噬细胞集落刺激因子(GMCSF)基因的质粒,24h后在同一部位注射FBL3肿瘤细胞破碎后的上清液,15d后用3×106个FBL3肿瘤细胞皮下接种攻击.结果]肿瘤的生长受到抑制,小鼠的生存时间明显延长.结论]基因枪途径局部接种含有GMCSF基因的质粒,可增强机体的抗肿瘤免疫,抑制肿瘤细胞的生长.
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| 关 键 词： | 淋巴瘤 基因 抑制 肿瘤 基因疗法 质粒 小鼠 |
| 文章编号： | 1000-1824(2005)01-0016-04 |
| 修稿时间： | 2004年11月25 |
Research of antitumor immunity in C 57 mice origin lymphoma with gene gun approach |
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| LI Rui-yong,CAI Chun-yu,JIN Wen-hao,ZHANG Shu-ren.Research of antitumor immunity in C 57 mice origin lymphoma with gene gun approach[J].Journal of Medical Science Yanbian University,2005,28(1):16-19. |
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| Authors: | LI Rui-yong CAI Chun-yu JIN Wen-hao ZHANG Shu-ren |
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| Institution: | LI Rui-yong1,CAI Chun-yu2,JIN Wen-hao1,ZHANG Shu-ren3 |
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| Abstract: | OBJECTIVE?To inoculate a mice GM-CSF expressing plasmid to C?57 mice with gene gun ~approach and induced antitumor immune effect.?METHODS?A mouse GM-CSF expressing plasmid (pcDNA_3?GM-CSF) was inoculated to C?57 mice with gene gun, and inoculated FBL-3 lymphoma cell crushing supernatant in situ after 24 hours. The mice were challenged by 3×106 parental tumor cells in 15 days.?RESULTS?Tumor development speed was reduced, survival time was remarkably delayed.?CONCLUSION?It could induce and enhance antitumor immune that inoculate C?57 mice pcDNA_3?GM-CSF with gene gun approach and with stand the parental tumor cell challenge effectively. |
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| Keywords: | lymphoma genes suppressor tumor gene therapy plasmids mice |
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