| 荧光光度法测定血清中美托洛尔浓度 |
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| 引用本文： | 仵文英,李兴华,雷建林,张阿惠. 荧光光度法测定血清中美托洛尔浓度[J]. 中国临床药学杂志, 1999, 0(Z1) |
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| 作者姓名： | 仵文英 李兴华 雷建林 张阿惠 |
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| 作者单位： | 西安医科大学第二附属医院药剂科 西安710004(仵文英，李兴华，雷建林)，西安医科大学药学系 西安710061(张阿惠) |
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| 摘 要： | 目的:建立荧光光度法测定美托洛尔血药浓度的方法.方法:用二氯甲烷作为提取溶剂,提取血清中美托洛尔.用蒸馏水定容后测定荧光强度.结果:血清中美托洛尔浓度在10～300ng/ml范围与荧光强度线性相关(c=0.877 2F 0.182 6,r=0.999 2).结论:美托洛尔血药浓度荧光光度法测定准确可靠、简便易行,可用于临床监测及药物动力学研究.
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| 关 键 词： | 荧光光度法 美托洛尔 血药浓度 |
Determination of metoprolol's concentration in blood serum by fluorescence method |
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| Wu Wenying,Zhang Ahui,Li Xinghua,et al. Determination of metoprolol's concentration in blood serum by fluorescence method[J]. Chinese Journal of Clinical Pharmacy, 1999, 0(Z1) |
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| Authors: | Wu Wenying Zhang Ahui Li Xinghua et al |
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| Affiliation: | Wu Wenying,Zhang Ahui,Li Xinghua,et al Department of Pharmacy,Second Affiliated Hospital of Xian Medical University,Xian 710004 |
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| Abstract: | AIM : To determine metoprolol's concentration in blood serum by a fluores-cence method. METHODS: Methylene chloride was used as extrative solvent to extract meto-prolol from blood serum. Distilled water was used to solution and then determine fluorescent intensity. RESULTS: MetoproloPs concentration in blood serum had a linear combinationwith the fluorescent intensity in the range of 10-300 ng/ml (c = 0. 887 2F 0. 182 6,r = 0. 999 2). CONCLUSION: The method designed is simple and feasible. It can be applied to the study of pharmacokinetics and to clinical monitoring of metoprolol levels in patients. |
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| Keywords: | fluorescence method metoprolol blood concentration |
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