| 抗Fas抗体诱导人肾小球系膜细胞凋亡 |
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| 引用本文： | 陈明,刘晓惠,黄颂敏,屈燧林,刘先蓉,许国章,刘聪. 抗Fas抗体诱导人肾小球系膜细胞凋亡[J]. 中国免疫学杂志, 1999, 15(3): 127-128 |
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| 作者姓名： | 陈明 刘晓惠 黄颂敏 屈燧林 刘先蓉 许国章 刘聪 |
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| 作者单位： | 1. 泸州医学院附属医院肾内科,泸州,646000
2. 华西医科大学附属第一医院肾病科,成都610041 |
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| 摘 要： | 研究抗Ｆａｓ抗体对人肾小球系膜细胞的致凋亡作用,探讨Ｆａｓ－ＦａｓＬ在肾脏损务中的作用。方法：常规方法分离,培养人肾小球系膜细胞,并传代鉴定,用不同浓度的抗Ｆａｓ抗体刺激４－６代Ｍｃｓ１６ｈ后,荧光染色观察Ｍｃｓ凋亡变化,二苯胺法和ＤＮＡ凝胶电泳方法定量和定性分析ＭｃｓＤＮＡ片段化变化。
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| 关 键 词： | 抗Fas抗体 系膜细胞 肾小球疾病 细胞凋亡 |
Anti-Fas antibody induce apoptosis in cultured human glomerular mesangial cells |
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| CHEN Ming,LIU Xiao Hui,HUANG Song Min et al. Anti-Fas antibody induce apoptosis in cultured human glomerular mesangial cells[J]. Chinese Journal of Immunology, 1999, 15(3): 127-128 |
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| Authors: | CHEN Ming LIU Xiao Hui HUANG Song Min et al |
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| Affiliation: | CHEN Ming,LIU Xiao Hui,HUANG Song Min et al.Department of Nephrology,the First University Hospital West China University of Medical Science,Chengdu 610041 |
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| Abstract: | Objective:To investigate the mechanism inducing apptosis of human glomerular mesangial cells (Mcs) by anti Fas antibody.Methods: Msc were cultured with anti Fas antibody (anti Fas Ab) at different concentrations for 16 hrs. Apoptosis of Mcs was evaluated and qualifed by acriding orange (AO) stained and DNA fragmentation assay. Results: Anti Fas antibody was found to be able to induce apoptosis in culture human masangial cells and the effect of anti Fas antibody inducing apoptosis was dose dependent.Conclusion:Fas and its ligand could play a an role in the injury of human glomerular cell. |
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| Keywords: | Fas FasL Anti Fas antibody Human mesangial cell |
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