| 基质金属蛋白酶-2对鼠心肌缺血再灌注间质损伤的影响 |
| |
| 引用本文： | 韩珂,杨辰垣,肖诗亮,刘成硅. 基质金属蛋白酶-2对鼠心肌缺血再灌注间质损伤的影响[J]. 实用医学杂志, 2005, 21(5): 452-456 |
| |
| 作者姓名： | 韩珂 杨辰垣 肖诗亮 刘成硅 |
| |
| 作者单位： | 430022,武汉市,华中科技大学同济医学院附属协和医院心外科 |
| |
| 摘 要： | 目的:探讨基质金属蛋白酶-2(MMP-2)在鼠缺血再灌注心肌的表达,及其与心肌间质损伤和心功能变化的关系。方法:Sprague-Dawley(SD)大鼠24只,随机分为对照组、缺血再灌注组和强力霉素组,每组8只。分别建立改良的Langendorrf离体心灌注模型,测定左室收缩峰压及压力微分,氯胺T法测定胶原含量,免疫组化法测定Ⅰ/Ⅲ胶原比例,酶谱法测定冠脉回流液不同时间点的MMP-2及其酶原(pro-MMP-2)。Westernblot检测心肌MMP-2的表达。结果:MMP-2活性在心肌缺血再灌注早期即明显升高,且其活性与心功能的恢复呈明显的相关性。心肌胶原含量和Ⅰ/Ⅲ比值较对照组均明显下降。强力霉素组表现出明显的保护作用。结论:MMP-2在心肌缺血再灌注间质损伤中起关键作用,应用MMP-2抑制剂可能成为保护心肌缺血再灌注损伤的新方法。
|
| 关 键 词： | 基质金属蛋白酶 缺血再灌注损伤 心肌 大鼠 |
The effect of matrix metalloproteinase-2 on interstitium of rat myocardium with ischemic reperfusion |
| |
| HAN Ke,YANG Chen-yuan,XIAO Shi-liang,LIU Cheng-gui. The effect of matrix metalloproteinase-2 on interstitium of rat myocardium with ischemic reperfusion[J]. The Journal of Practical Medicine, 2005, 21(5): 452-456 |
| |
| Authors: | HAN Ke YANG Chen-yuan XIAO Shi-liang LIU Cheng-gui |
| |
| Affiliation: | HAN Ke,YANG Chen yuan,XIAO Shi liang,LIU Cheng gui. Department of Cardiovascular Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China |
| |
| Abstract: | Objective To assess the expression of matrix metalloproteinase on rat myocardium with ischemic reperfusion(I/R) and effect on mechanical function recovery of heart and relationship between its expression and interstitium injury. Methods Twenty four SD rats were randomly divided into three groups, control group, ischemic reperfusion group and doxycyline group. Each group includes 8 rats. To establish isolated rat heart modified Langendorrf model retrograde perfusion. The left ventricular peak systolic pressu... |
| |
| Keywords: | Matrix metalloproteinase Ischemia reperfusion Myocardium Rat |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《实用医学杂志》浏览原始摘要信息 |
|
点击此处可从《实用医学杂志》下载全文 |
|
