碱性成纤维细胞生长因子影响痴呆大鼠海马神经发生

目的探讨碱性成纤维细胞生长因子(bFGF)对海马伞切断造成的阿尔茨海默氏病(AD)模型大鼠海马齿状回神经发生的影响.方法 AD处理组及AD对照组大鼠左侧海马伞切断制造阿尔茨海默病模型;正常对照组注射生理盐水.AD处理组造模后每天侧脑室注射bFGF共7 d;AD对照组及正常对照组同时间注射生理盐水.BrdU标记增殖细胞.TUNEL方法标记DNA片段,原位检测凋亡细胞.计数海马齿状回BrdU阳性细胞与凋亡细胞数.结果 AD处理组大鼠与AD对照组大鼠相比,海马齿状回BrdU阳性细胞增加非常显著(P＜0.01),而凋亡细胞差异不显著(P＞0.05),AD对照组大鼠与正常对照组大鼠相比,海马齿状回BrdU阳性细胞数及凋亡细胞差异均不显著(P＞0.05).结论 bFGF可刺激AD模型大鼠海马神经发生.是治疗AD等神经缺损性疾病有希望的一种神经生长因子.

Effect of basic fibroblast growthfactor onneurogenesis in the dentate gyrus of AD model rat with unilateral timb ria -fornix transection

Objective:To study the effect of basic fibroblast growth factor on neurogenesis in brain of AD model rat with unilateral fimbria-fornix transection.Methods:We developed the AD model through unilateral fimbria-fomix transection. AD model rats received bFGF or saline by infusion into the cerebroventricular space every day after being lesioned. After 7 days bFGF infusion, 5-BrdU was used to label the proliferation cells after bFGF infusion. TUNEL was used to label the apoptotic cell .Then we count the positive cells labeled for 5-BrdU or TUNEL in the dentate gyrus.Results:Comparison to AD control group, the number of 5-BrdU positive cells in the dentate gyms of AD treatment group was increased significantly .But the number of TUNEL positive cells was no difference from that of the AD control group. The number of 5-BrdU positive ceils and the number of TUNEL positive cells in the dentate gyrus of AD control group were no difference from that of the normal control group'.Conclusions:It is showed that bFGF can acceleration the neurogenesis in the dentate gyms of AD model rat with unilateral fimbfia-fomix transection.