黄芪注射液在整体抗疲劳实验中对小鼠骨骼肌HK的影响

目的：研究不同剂量的黄芪注射液对小鼠骨骼肌的抗疲劳效应及对肌己糖激酶活性的影响.方法：记录小鼠腓肠肌收缩曲线,测定肌疲劳的时间,检测腓肠肌己糖激酶的活性.结果：与对照组比较,各黄芪注射液组小鼠的肌疲劳的时间均延长,差异具有显著性（P＜0.05）.对照组、黄芪注射液1.2g·kg-1、1.6g·kg-1和2.0g·kg-1组HK活性分别为：5.23±0.87、3.21±1.31、4.68±1.36和4.37±1.52.与对照组比较,黄芪注射液1.2g·kg-1组的HK活性降低,差异具有显著性（P＜0.05）;黄芪注射液1.6g·kg-1和2.0g·kg-1组的HK活性降低,但差异不具有显著性（P＞0.05）.结论：黄芪注射液1.2g·kg-1组具有延缓肌疲劳时间,可适度抑制骨骼肌HK活性而产生抗骨骼肌疲劳效应的作用.

Effect of astragalus membranaceus injection on HK in overall anti-fatigue mice

Objective： To investigate the anti-fatigue effect and hexokinase activity of astragalus membranaceus injection with different doses in mice skeletal muscle. Methods： The contractive tension of gastrocnemius muscle was recorded, the time of muscular fatigue was measured, and the activity of hexokinase was detected after the injection of the drug. Results： Compared with the control group, each dose of astragalus membranaceus injection could delay the time of muscular fatigue significantly （P〈0. 05）. HK activity of mice in control group, astragalus membranaceus injection at the dose of 1.2, 1.6 and 2. 0 g·kg-1 groups were 5.23±0. 87, 3.21±1. 31, 4. 68±1.36 and 4. 37±1.52, respectively. Compared with the control group, HK activity reduced more obviously in the astragalus membranaceus injection 1.2 g ·kg-1 group （P〈0. 05）. Conclusion： Astragalus membranaceus injection at the dose of 1.2 g·kg-1 could delay the time of muscular fatigue by inhibiting the activity of HK markedly.