核工业某厂放射工作人员与非放射工作人员的MDM2基因表达调查与分析

目的 探讨核工业某厂放射工作人员与非放射工作人员辐射敏感基因表达的差异。 方法 采集了3名健康人外周血进行60Co γ射线照射，建立辐照后鼠双微体基因2（MDM2）表达的剂量-效应关系，抽取核工业某厂96名工作人员（49名放射工作人员、47名非放射工作人员）外周血，利用实时荧光定量PCR技术检测放射工作人员外周血中MDM2基因的表达情况。 结果 在0~2 Gy剂量范围内，MDM2的表达水平随照射剂量增加而上升。某厂96名工作人员外周血MDM2基因表达水平的检测分析结果表明，非放射工作人员年龄因素对外周血MDM2基因表达无影响（F=2.11，P＞0.05）；放射工作人员外周血MDM2基因表达与非放射工作人员相比，差异具有统计学意义（t=7.78，P＜0.05）。 结论 电离辐射可以诱导人离体外周血MDM2基因表达改变，且基因表达与照射剂量存在一定的剂量相关性，MDM2有望成为核工业放射工作人员健康调查的敏感指标。

Investigation and analysis of MDM2 expression level in radiation-exposed workers and non-radiation-exposed workers

Objective To investigate the difference of radiation-sensitive gene expression between radiation-exposed and non-radiation-exposed workers in a nuclear industry factory. Methods Peripheral blood samples from 3 healthy volunteers were exposed to 60Co γ-rays at different doses. Exposure of murine double minute 2(MDM2) mRNA expression to ionizing radiation was observed. MDM2 gene expression levels were detected in peripheral blood from 96 workers(i.e., 49 radiation-exposed workers and 47 non-radiation-exposed workers) in a nuclear industry factory by using real-time PCR. Results The MDM2 expression levels of peripheral blood samples from 3 healthy volunteers exposed to 60Co γ-rays increased with an increased dose of 0 Gy to 2 Gy. Moreover, the relationship between the ratio of MDM2 mRNA/β-actin and irradiation dose represents certain line correlation, which was fit by Origin 7.5 software. The detection and analysis results of MDM2 gene expression levels in nuclear factory workers showed no statistically significant difference between different age groups and MDM2 gene expression levels in the peripheral blood of non-radiation-exposed equilibrium distribution of staff(F=2.11, P>0.05). Therefore, age factor had no effect on MDM2 gene expression. The MDM2 gene in the peripheral blood of radioactivity staff was statistically significant to radiation-exposed workers(t=7.78, P < 0.05). Conclusions Ionizing radiation can induce changes in gene expression from human peripheral blood, and this process is dose-related. MDM2 gene expression levels may be a new radiosensitive index in health examination survey in the future.