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| 人乳牙牙髓干细胞的体外培养和鉴定 | |
| 引用本文： | 许诺,陈柯,申元源. 人乳牙牙髓干细胞的体外培养和鉴定[J]. 南方医科大学学报, 2009, 29(3): 479 |
| 作者姓名： | 许诺 陈柯 申元源 |
| 作者单位： | 1. 南方医科大学南方医院口腔科,广东,广州,510515 2. 南方医科大学南方医院惠侨科口腔科,广东,广州,510515 |
| 摘 要： | 目的 从健康儿童滞留的乳牙牙髓组织中分离出乳牙牙髓干细胞(SHED),体外培养观察,研究其生物学特性.方法 用酶消化法培养SHED,观察细胞形态、克隆形成能力,以免疫组化和流式细胞技术检测多种抗原表达情况及细胞周期,并进行体外分化实验,诱导SHED形成脂肪组织及矿化结节.结果 以酶消化法分离培养所得的细胞多形性明显,克隆形成效率为16~18/103细胞,其中富含有表达STRO-1表型的细胞.在一定条件下,所培养细胞具有定向分化能力.结论 以酶消化法分离培养获得的细胞中,包含自我更新能力强、有一定分化能力的干细胞,即SHED. |
| 关 键 词： | 乳牙 牙髓 干细胞 细胞培养 表型 细胞分化 |
Isolation and identification of stem cells derived from human exfoliated deciduous teeth |
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| XU Nuo,CHEN Ke,SHEN Yuan-yuan. Isolation and identification of stem cells derived from human exfoliated deciduous teeth[J]. Journal of Southern Medical University, 2009, 29(3): 479 | |
| Authors: | XU Nuo CHEN Ke SHEN Yuan-yuan |
| Affiliation: | XU Nuo1,CHEN Ke2,SHEN Yuan-yuan1 Department of Stomatology1,Department of Stomatology,Huiqiao Department2,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China |
| Abstract: | Objective To isolate and identify stem cells from human exfoliated deciduous teeth(SHED).Methods Human pulp tissue were dissected and digested to obtain the single cell suspension.The cell morphology was observed and the clonality of the obtained cells was assessed.The phenotype of the cells was detected by immunohistochemistry and flow cytometry(FCM),and the cell cycle was analyzed.The in vitro differentiation of the cells into adipose tissue and formation of mineralization nodules were evaluated.Results C... |
| Keywords: | deciduous teeth dental pulp stem cells cell culture phenotype differentiation |
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