急性白血病与可溶性肿瘤坏死因子受体的相关性研究

应用ELISA法检测了60例急性白血病（AL）患者及36例正常对照者的血清可溶性肿瘤坏死因子受体（sTNFR-Ⅰ和sTNFR-Ⅱ）及血清肿瘤坏死因子α（TNFα）。结果显示，①初发未治组及复发难治组血清sTNFR-Ⅰ和sTNFR-Ⅱ浓度均明显高于正常对照组（P＜0.01）；骨髓缓解组血清sTNFR浓度与正常对照组无明显差异。AL患者与对照组比较血清TNF-α水平明显增高（P＜0.001）；②血清TNF-α水平与sTNFR-Ⅰ呈显著性正相关（r＝0.684，P＜0.01），与sTNFR-Ⅱ无相关性（r＝0.310，P＞0.05）；血清sTNFR-Ⅰ、sTNFR-Ⅱ水平与外周血幼稚细胞数呈显著正相关（r＝0.748，P＜0.001；r＝0.384，P＜0.05）；③30例初发未治者治疗前血清sTNFR-Ⅰ＜0.89μg／L、sTNFR-Ⅱ＜1.43μg／L者的BMR率（70.0％、61.5％）明显高于sTNFR-Ⅰ≥0.89μg／L、sTNFR-Ⅱ≥1.43μg／L者的BMR率（χ

Relationship between acute leukemia and soluble tumot hecrosis factor

Serum tumor necrosis factor（TNFα），serum soluble tumor necrosisfactor receptor Ⅰ（sTNFR-Ⅰ）and soluble tumor necrosis factor receptor（sTNFR-Ⅱ）were detected with enzyme-link immunosorbent assay in 60 acute leukemia（AL） cases and 36 health subjects.The results showed：①Serum levels of sTNFR-Ⅰ and sTNFR-Ⅱ in 30 untrearted AL patients and 14 relapsed／refractory AL patients were significantly higher than those in controls（P＜0.001and P＜0.001），but in 16 bone marrow remission（BMR）AL patients its were not different from those in controls（P＞0.05）.Serum levels of TNFα in AL patients were significantly higher than those in controls（P＜0.001）.②There were remarkably positive relationship between serum TNFα and sTNFR-Ⅰ levels（r＝0.684，P＜0.01），two sTNFRs and peripheral blood blasts（r＝0.748，P＜0.001；r＝0.384，P＜0.05）.③The BMR rate was higher in those serum sTNFR-Ⅰ＜0.89μg／L than that in those sTNFR-Ⅰ≥0.89μg／L（χ２＝10.80，P＜0.01）．④Logistic regression analysis showed that serum sTNFR-Ⅰ＜2.0μg／L and peripheral blood blasts＜10×10９/L were associated with BMR.Those indicated the enhanced serum sTNFRs levels in AL might have relation to leukemic cells，course and drug response and the serum sTNFRs were an index of predicting prognosis.