王氏抗肝纤方治疗肝纤维化的实验研究

目的：观察王氏抗肝纤方对肝纤维化的治疗作用及其作用机制。方法：采用二甲基亚硝胺诱导肝纤维化模型，从组织学改变观察王氏抗肝纤方的疗效，以γ干扰素及膈下逐阏汤作为对照组。结果：王氏抗肝纤方对肝纤维化大鼠能显著降低血清ＡＬＴ、ＡＳＴ及肝组织Ｈｙｐ含蛳，减少肝组织中ＣｏｌＩ、ＣｏｌⅣ的沉积和α－ＳＭＡ的表达，提高肝组织间质胶原酶活性。其作用同γ干扰素而明显优于膈下逐瘀汤。结论：王氏抗肝纤方具有较好的抗肝纤

Experimental Study on the Therapeutic Effect of Wang's Anti-fibrosis Decoction

Aim: To study the pharmacological actions of Wang's Anti-fibrosis decoction on experimental liver fibrosis in Rats. Methods t Using dimethylnitrosamine (DMN) produced rat model of liver fibrosis. observed therapeutic effect of Wang's anti-fibrosis decoction on alteration of histology. Interferon gamma (IFN-T) and Gexiazhuyu decoction were used in control group. Results: Wang's anti-fibrosis decoction lowered the serum level of ALT, AST and Hyp in liver stissue. Image analysis indicated significant attenuation of collagen type I , IV and a-smooth muscle actin (a-SMA) content after Wang's anti-fibrosis decoction treatment. The hepatic interstitial collagenase activity was raised. No obvious different from IFN-T group but was better than Gexiazhuyu decoction group. Conclusions: These results suggested that Wang's anti-fibrosis decoction have showed better anti-hepatofibrosis effect in experimental rats. The proliferation of collagenous fibers was restrained and the decomposition was impelled by raise the interstitial collagenase activity.