血浆中APC基因甲基化检测在早期肺癌诊断中的应用

目的 研究血浆DNA中APC基因启动子甲基化与肺部实体瘤良、恶性的关系,确定血浆中APC基因甲基化检测对早期肺癌诊断的意义.方法 以92例CT检查发现肺部实体瘤(直径≤2 cm),并在CT引导下行肺部穿刺患者和23例健康人作为研究对象,收集血浆样本,利用磁珠法提取DNA,行亚硫酸氢盐化学修饰后,采用针对APC基因启动子区的甲基化引物和探针,以荧光定量MSP法(TaqMan探针)检测APC基因的甲基化.结果 92例肺部实体瘤患者经病理诊断,有58例确诊为肺癌、31例为肺部良性疾病、3例未明确诊断.58例肺癌患者中有11例(19%)测出APC基因启动子区甲基化阳性,31例肺部良性疾病、3例未明确诊断和23例健康对照组均为阴性.结论 检测血浆DNA中APC基因启动子甲基化有助于早期肺癌的诊断.

Detection of APC gene promoter methylation in plasma of early lung cancer

Objective To detect the methylation of APC gene promoter in plasma from lung tumor patients and determine its meaning in early lung cancer diagnosis.Methods Plasma was collected from 92 patients with lung solid tumor diagnosed by CT-guided punctuation and 23 healthy volunteers.All the 92 patients,in whom 58 were lung cancer,31 were benign lung disease and 3 were indefinite diagnosis,were confirmed by histology.Plasma DNA was extracted by Chemagic Viral DNA/RNA kit.Real-time MSP was used to detect the methylation of APC gene promoter after bisulfite modification.Results In 58 lung cancer patients 11 cases were positive(11/58,19%),but no positive case was found in the other patients(31 benign lung disease,3 indefinite diagnosis) or healthy controls.Conclusions The detection of APC gene promoter methylation in plasma by the real-time MSP may be useful in the early diagnosis of lung cancer.