不同穴组针刺对抑郁大鼠下丘脑-垂体-肾上腺轴相关因子及血清脑源性神经营养因子的影响

目的:筛选针刺抗抑郁穴位,并探讨其可能的作用机制。方法:SD只大鼠随机分为正常组、模型组、针刺一组、针刺二组、西药组,每组10只。除正常组大鼠10只同笼饲养外,其余各组大鼠单笼孤养并进行28d的慢性轻度不可预见性应激刺激。造模同时,针刺一组取"印堂"穴和"百会"穴,针刺二组选用"印堂""百会""风池""肾俞"穴进行针刺并留针30min,西药组予氟西汀0.18mg/kg灌胃,均每日1次,干预28d。于实验开始前及结束后进行旷场实验,实验结束后采用ELISA法检测各组大鼠脑垂体促肾上腺皮质激素(ACTH)、海马5羟色胺(5-HT)、肾上腺皮质醇(CORT)、血清脑源性神经营养因子(BDNF)含量。结果:模型组大鼠水平运动与垂直运动次数均显著少于正常组(P0.01,P0.05);与模型组比较,针刺一组、针刺二组、西药组水平运动与垂直运动次数均提高(P0.01)。各组大鼠垂体ACTH水平比较,差异无统计学意义(P0.05)。与正常组比较,模型组大鼠海马5-HT、血清BDNF水平显著下降,肾上腺CORT水平显著上升(P0.01);针刺一组、针刺二组、西药组大鼠海马5-HT、血清BDNF水平均较模型组显著上升,肾上腺CORT水平均较模型组显著下降(P0.05,P0.01)。各治疗组之间比较,各指标差异均无统计学意义(P0.05),但针刺二组显示出更佳的改善趋势。结论:针刺可有效改善抑郁模型大鼠的自发运动减少,其机制可能与上调海马5-HT、血清BDNF水平,下调肾上腺CORT水平有关,且针刺"百会""印堂""风池""肾俞"显示出疗效最佳的趋势。

Effect of Acupuncture Stimulation of Different Acupoint Groups on Levels of Stress Hormones and Serum Brain-derived Neurotrophic Factor in Depression Rats

Objective To screen the effective acupoints for anti-depression in depression rats and to explore the mechanisms of acupuncture for relieving depression.Methods Fifty rats were randomly divided into normal control,model,"Yintang"(EX-HN 3)-"Baihui"(GV 20,2-acupoints),EX-HN 3-GV 20-"Fengchi"(GB 20)-"Shenshu"(BL 23,4-acupoints)and medication groups,with 10 rats in each group.The depression model was established by unpredictable chronic mild stress(UCMS)for28days.For rats of the 2-acupoints and 4-acupoints groups,EX-HN 3and GV 20,and EX-HN 3,GV 20,GB 20 and BL 23 were punctured with filiform needles respectively before performing mild stress every time.The acupuncture needles were retained for30 min during each intervention and the treatment was conducted once daily for 28 days.The rats of the medication group were treated by intragastric administration of Fluoxetine(0.18mg/kg)once a day for 28 days.The rats' anxiety-like behavior(rearing and crossing times)was detected by open-field test.The contents of adrenocorticotropic hormone(ACTH)in the pituitary,5-hydroxytryptamine(5-HT)in the hippocampus,the cortisol(CORT)in the adrenal gland,and the brain derived neurotrophic factor(BDNF)in the serum were examined by ELISA.Results Compared to the normal control group,the numbers of both rearing and crossing motions in the model group were significantly decreased(P<0.01,P<0.05),while in comparison with the model group,the rearing and crossing numbers of rats in the 2-acupoints and 4-acupoints and medication groups were significantly increased(P<0.01).ELISA showed that after modeling,the content of adrenal CORT was significantly increased(P<0.01),and those of hippocampal 5-HT and serum BDNF were obviously down-regulated in the model group(P<0.01).After the treatment,the adrenal CORT levels in the three intervention groups were notably down-regulated,and hippocampal 5-HT and serum BDNF evidently up-regulated in these 3intervention groups(P<0.05,P<0.01).No marked changes were found in the pituitary ACTH contents of the model and 3intervention groups(P>0.05),and no significant differences were shown among the three intervention groups in the levels of the aforementioned 6indexes(P>0.05).Conclusion Acupuncture intervention can effectively improve the unprompted activates of the depression rats,which may be related to its effects in up-regulating hippocampal 5-HT and serum BDNF levels,and in down-regulating adrenal CORT content.