河南省HIV流行毒株env膜蛋白基因C2-V3区序列特征和亚型研究

目的 了解河南省内艾滋病病毒Ⅰ型(HIV-1)流行株的亚型及序列变异特征。方法 用套式聚合酶链反应(nested-PCR)，对河南省内28份HIV-1感染者外周血单个核细胞(PBMc)中前病毒脱氧核糖核酸(DNA)的膜蛋白基因进行扩增，并对C2-V3及其邻区350～450个核苷酸序列进行测定和分析。结果 28份样品间的基因离散率为5．72％，与流行于云南省的(泰国B)毒株基因距离为5.80％，与欧美B亚型基因距离为18．05％，而与其它A、C、D、F、G、H、J、O等亚型的基因距离均在24％以上。系统树分析显示，28份样本与泰国B亚型聚在一起而远离其它国际亚型。对于其V3环四肽序列的分析表明，具有泰国B亚型基因型GPGQ的8例，占28．57％；具有欧美B亚型基因型GPGR的13例，占46．4％。结论 河南省流行的HIV株属B‘亚型，其毒株来自与泰国接壤的云南省，在河南省流行的时间约在6～10年，其V3环顶端四肽序列特征以GPGR为主。

Study on subtype and sequence of the C2-V3 region of env gene among HIV-1 strains in Henan province

objective To investigate the subtype distribution and sequence characteristics of HIV-1 strains prevalent in Henan province.Methods HIV-1 env gene was amplified by PCR from uncultured peripheral blood mononuclear cells(PBMCs)obtained from 28 HIV-1 seropositive individuals in Henan in 2002.The C 2-V 3 region(about 450bp)was sequenced.Results The innergroup gene divergence of 28 sample is 5.72%,and 5.80% with reference strains of Thailand B subtype from Yunnan,18.05% with North America B,over 24% with other subtypes including A,C,D,F,G,H,J,O.Phylogenetic tree analysis showed that 28 Henan B strains clustered closely to Thailand B and were far from other international subtypes.The analysis of V 3 loop amino sequence of 28 Henan B strains revealed that V 3-tip motif of 8 samples(28.57%)was GPGQ of Thailand B subtype gene,12 samples(42.86%)GPGR of Europe and America B subtype gene.Conclusion These findings suggest the epidemic of subtype B of HIV-1 in Henan province and it is closely related to those identified in Yunnan province.The data showed that these viruses existed for about 6-10 years.V 3 tip motifs of the HIV-1 isolates were mainly GPGR.