丹参素对小鼠外周血造血干细胞动员作用的研究

目的:探讨丹参素对小鼠外周血造血干细胞的动员作用及其对小鼠外周血细胞和骨髓基质细胞黏附分子的影响。方法:30只BALB/c小鼠随机分为三组:丹参素组、G-CSF组、生理盐水组,腹腔注射d1～d7,每日1次,第2～8天,采用外周血WBC和MNC计数、流式细胞术、造血祖细胞体外培养、免疫细胞化学等检测各组给药后对外周血WBC、MNC、CD34+细胞、CD49d阳性细胞、CFU-GM、CFU-MK、CFU-E的产率及小鼠骨髓基质细胞VCAM-1阳性细胞百分率的影响。结果:丹参素组给药第7天外周血WBC、MNC数量达到高峰,分别为给药前的3倍和3.5倍;丹参素组外周血CD34+、CD49d阳性细胞及骨髓基质细胞VCAM-1阳性细胞百分率分别为(1.03±0.24)%、(12.59±2.64)%和(50.86±8.77)%,均明显高于生理盐水组(P<0.05);其CFU-GM、CFU-MK和CFU-E产率分别为(14.90±2.88)%、(12.50±4.06)%和(16.10±6.36)%,均明显高于生理盐水组(P<0.01)。结论:丹参素对小鼠外周血造血干细胞有一定的动员作用,且这一作用可能与其上调小鼠外周血细胞和骨髓基质细胞黏附分子的表达有关。

Mobilization of Tanshinol on Peripheral Blood Hematopoietic Stem Cells in Mice

Objective：To study tanshinol for mobilizing peripheral blood hematopoietic stem cells and the effect of its on the expression of adherent molecule of peripheral blood hematopoietic stem cells and bone marrow cells in BABL/c mice.Methods：Thirty BABI./c mice were divided into three groups at random （n=10 each）：From day 1 to 7,mice were intraperitoneally injected with tanshinol （Group Tanshinol） and rhG-CSF （Group G-CSF） respectively,normal control with equivalence saline.The effects of the numbers of WBC and MNC,the percentage of CD34^＋ cell and CD49d positive cell and the yields of CFU-GM,CFU-MK,CFU-E in peripheral and the percentage of VCAM-1 positive cells in bone marrow stromal cells of the mice were observed by means of WBC and MNC counts, flow cytometry, hematopoietic cell culture in vitro and immunoeytochemistry.Results：7 days after be treated of group tanshinol, the numbers of WBC and MNC reached the maximum, which were 3 and 3.5 times that of the NS group respectively;the percentage of CD34^＋ cells and CD49d positive cells in peripheral blood and VCAM-1 positive cells in bone marrow stromal cells in group tanshinol was （1.03± 0.24）%, （12.59± 2.64）% and（50.86±8.77）% respectively,were markedly higher than in NS group（P 〈0.05）; the yields ofCFU-GM ,CFU-MK and CFU-E in group oftanshinol was （14.90± 2.88）% ,（12.50±4.06）% and （16.10± 6.36）% respectively,were markedly higher than in NS group （P 〈0.01）.Conclusion：Tanshinol could mobilize hematopoietic stem cells into peripheral blood .And this role may be related to the increase adhesion molecule expression in peripheral blood cells and mouse bone marrow stromal cell.